Plant Virology Protocols

From Virus Isolation to Transgenic Resistance

  • Gary D. Foster
  • Sally C. Taylor

Part of the Methods in Molecular Biology™ book series (MIMB, volume 81)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Bradley I. Hillman
      Pages 3-12
    3. Ron S. S. Fraser
      Pages 13-24
    4. Eric D. Miller, Cynthia Hemenway
      Pages 25-38
  3. Virus Isolation, RNA Extraction, and Location of Coat Protein Genes

    1. Front Matter
      Pages 39-39
    2. Kenneth E. Palmer, Wendelin H. Schnippenkoetter, Edward P. Rybicki
      Pages 41-52
    3. Simon N. Covey, Rob J. Noad, Nadia S. Al-Kaff, David S. Turner
      Pages 53-63
    4. Ichiro Uyeda, Bong-Choon Lee, Yuko Ando, Haruhisa Suga, Yun-Kun He, Masamichi Isogai
      Pages 65-75
    5. Diane M. Lawrence, Andrew O. Jackson
      Pages 99-106
    6. Salah E. Bouzoubaa
      Pages 107-114
    7. Sally C. Taylor
      Pages 115-121
    8. Sean N. Chapman
      Pages 123-129
    9. Mounir G. AbouHaidar, Huimin Xu, Kathleen L. Hefferon
      Pages 131-143
    10. Gary D. Foster
      Pages 145-150
    11. Philip H. Berger, Patrick J. Shiel
      Pages 151-160
    12. Sylvie German-Retana, Thierry Candresse, Jean Dunez
      Pages 161-170
    13. Deyin Guo, Edgar Maiss, Günter Adam
      Pages 171-181
    14. Jozef Julian Bujarski
      Pages 183-188

About this book

Introduction

Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology.

Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.




Editors and affiliations

  • Gary D. Foster
    • 1
    • 2
  • Sally C. Taylor
    • 2
  1. 1.Plant Virology Group School of Biological SciencesUniversity of BristolUK
  2. 2.Department of BotanyUniversity of LeicesterUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033856
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-385-6
  • Online ISBN 978-1-59259-566-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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