Epitope Mapping Protocols

  • Glenn E. Morris

Part of the Methods in Molecular Biology™ book series (MIMB, volume 66)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Glenn E. Morris
    Pages 1-9
  3. Frederick A. Saul, Pedro M. Alzari
    Pages 11-23
  4. Giuseppe A. Molinaro, William C. Eby
    Pages 39-46
  5. Socrates J. Tzartos
    Pages 55-66
  6. N. Martin Young, Raymond P. Oomen
    Pages 77-83
  7. Maria R. Mazzoni, Nikolai O. Artemyev, Heidi E. Hamm
    Pages 109-120
  8. Glenn E. Morris
    Pages 121-127
  9. Yingming Zhao, Brian T. Chait
    Pages 129-136
  10. Stuart J. Rodda, N. Joe Maeji, Gordon Tribbick
    Pages 137-147
  11. Ronald Frank, Heike Overwin
    Pages 149-169
  12. Alexander Pereboev, Glenn E. Morris
    Pages 195-206
  13. Lin-Fa Wang
    Pages 207-220

About this book


Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo­ gists from a wide range of disciplines in which antibodies are used as molecu­ lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune diseases, or in defining protein topology in intact cells or organelles, to mention only a few of the possibilities. The aim of Epitope Mapping Protocols is to provide both a useful range of alternative practical methods for the experienced mapper and a fairly com­ prehensive introduction for someone embarking on antibody production and mapping for the first time. Contributors were encouraged to illustrate their protocols with results from their own research and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptide libraries; and the third group of eleven requires antigen expressed from recombinant DNA. Within this last group, methods more likely applicable to conformational epitopes are placed first.

Editors and affiliations

  • Glenn E. Morris
    • 1
  1. 1.The North East Wales InstituteWrexhamUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033759
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-375-7
  • Online ISBN 978-1-59259-552-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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