Plant Gene Transfer and Expression Protocols

  • Heddwyn Jones

Part of the Methods in Molecular Biology™ book series (MIMB, volume 49)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Agrobacterium-Mediated Transformation

  3. Direct Gene Transfer

  4. Use of Reporter Genes

    1. Gillian A. Hull, Martine Devic
      Pages 125-141
    2. Michael R. Davey, Nigel W. Blackhall, J. Brian Power
      Pages 143-148
    3. Ian S. Curtis, J. Brian Power, Michael R. Davey
      Pages 149-159
  5. Study of Gene Organization by Southern Blotting and Inverse PCR

    1. Paolo A. Sabelli, Peter R. Shewry
      Pages 161-180
  6. RNA Techniques for Studying Gene Expression

    1. Rod J. Scott
      Pages 197-202
    2. Rod J. Scott
      Pages 203-206
    3. Rod J. Scott
      Pages 207-211
    4. Paolo A. Sabelli, Peter R. Shewry
      Pages 213-228
    5. John Gatehouse, Andrew J. Thompson
      Pages 229-238
    6. Craig G. Simpson, John W. S. Brown
      Pages 239-247
    7. Craig G. Simpson, John W. S. Brown
      Pages 249-256
    8. Craig G. Simpson, John W. S. Brown
      Pages 257-269
    9. Richard Cooke, Paul Penon
      Pages 271-289
    10. Shirley R. Burgess
      Pages 301-316
    11. Frederica L. Theodoulou, Anthony J. Miller
      Pages 317-340
    12. Laurence J. Trueman
      Pages 341-354
  7. Techniques for Studying Chloroplast Gene Expression

    1. Johnathan A. Napier, Simon A. Barnes
      Pages 355-360
    2. Johnathan A. Napier
      Pages 361-367
    3. Johnathan A. Napier
      Pages 369-376
  8. Techniques for Studying Mitochondrial Gene Expression

    1. Stefan Binder, Lutz Grohmann
      Pages 377-381
    2. Lutz Grohmann
      Pages 391-397
  9. Immunological Detection of Proteins

    1. Peter R. Shewry, Arthur S. Tatham, Roger J. Fido
      Pages 399-422
    2. Roger J. Fido, Arthur S. Tatham, Peter R. Shewry
      Pages 423-437
    3. E. N. Clare Mills, Geoffrey W. Plumb, Michael R. A. Morgan
      Pages 439-451
  10. Back Matter
    Pages 463-466

About this book


The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science. First, it has allowed investigation, using reporter genes, into the transcriptional regulation of plant genes—a key to the under­ standing of the biochemical basis of growth and development in plants. Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g. , homeotic genes) whose gene products control the normal pattern of growth and differentia­ tion of plants. Third, overproducing foreign or endogenous proteins in plants can often lead to a better understanding of biochemical and physiological processes. Fourth, gene transfer technology has allowed the improvement of plant agricultural productivity. For example, plants have been engineered with improved viral resistance or the ability to withstand herbicide attack, therefore allowing a more effective use of herbicides to kill weeds. Fifth, there have been recent successes that demonstrate the potential use of plants as biotechnological chemical factories. For example, it is possible to use plants in the production of human antibodies and antigens of medical importance. It has been demonstrated recently that plants can be engineered to produce modified oils and even plastics! This paves the way to redirect agriculture from the production of surplus foods to the production of bio­ technological products of industrial importance.

Editors and affiliations

  • Heddwyn Jones
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1995
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-321-4
  • Online ISBN 978-1-59259-536-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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