Patch-Clamp Applications and Protocols

  • Alan A. Boulton
  • Glen B. Baker
  • Wolfgang Walz

Part of the Neuromethods book series (NM, volume 26)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Richard A. Levis, James L. Rae
    Pages 1-36
  3. Harald Sontheimer
    Pages 37-73
  4. Owen P. Hamill, Don W. McBride Jr.
    Pages 75-87
  5. C. G. Nichols, M. B. Cannell, A. N. Lopatin
    Pages 89-121
  6. John M. Tang, E. N. Giuandt, R. S. Eisenberg
    Pages 123-139
  7. Norio Akaike
    Pages 141-154
  8. Wolfgang Walz
    Pages 155-171
  9. J. H. Caldwelland, R. L. Milton
    Pages 173-192
  10. Bertrand Lambolez, Etienne Audinat, Pascal Bochet, Jean Rossier
    Pages 193-231
  11. T. D. Plant, J. Eilers, A. Konnerth
    Pages 233-258
  12. T. G. Smart, B. J. Krishek
    Pages 259-305
  13. Back Matter
    Pages 307-316

About this book

Introduction

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.

Editors and affiliations

  • Alan A. Boulton
    • 1
  • Glen B. Baker
    • 2
  • Wolfgang Walz
    • 1
  1. 1.Uniuersity of SaskatchewanSaskatoonCanada
  2. 2.Unioersity of AlbertaEdmontonCanada

Bibliographic information

  • DOI https://doi.org/10.1385/0896033112
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-311-5
  • Online ISBN 978-1-59259-631-7
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • About this book
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