Electroporation Protocols for Microorganisms

1. Transformation of Lactobacillus by Electroporation

   • Thea W. Aukrust, May B. Brurberg, Ingolf F. Nes

   Pages 201-208

2. Electrotransformation of Staphylococci

   • Jean C. Lee

   Pages 209-216

3. Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine

   • Brett D. Shepard, Michael S. Gilmore

   Pages 217-226

4. Introduction of Recombinant DNA into Clostridium spp.

   • Mary K. Phillips-Jones

   Pages 227-235

5. Electroporation of Mycobacteria

   • T. Parish, N. G. Stoker

   Pages 237-252

6. Electrotransformation of the Spirochete Borrelia burgdorferi

   • D. Scott Samuels

   Pages 253-259

7. Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation

   • Lisa Stowers, James Gautsch, Richard Dana, Merl F. Hoekstra

   Pages 261-267

8. Ten-Minute Electrotransformation of Saccharomyces cerevisiae

   • Martin Grey, Martin Brendel

   Pages 269-272

9. Electroporation of Schizosaccharomyces pombe

   • Mark T. Hood, C. S. Stachow

   Pages 273-278

10. Gene Transfer by Electroporation of Filamentous Fungi

    • M. Kapoor

    Pages 279-289

11. Transformation of Candida maltosa by Electroporation

    • Dietmar Becher, Stephen G. Oliver

    Pages 291-302

12. Electroporation of Physarum polycephalum

    • Timothy G. Burland, Juliet Bailey

    Pages 303-320

13. Electroporation of Dictyostelium discoideum

    • David Knecht, Ka Ming Pang

    Pages 321-330

14. Gene Transfer by Electroporation in Tetrahymena

    • Jacek Gaertig, Martin A. Gorovsky

    Pages 331-348

15. Transfection of the African and American Trypanosomes

    • John M. Kelly, Martin C. Taylor, Gloria Rudenko, Pat A. Blundell

    Pages 349-359

16. Electroporation in Giardia lamblia

    • A. L. Wang, Tiina Sepp, C. C. Wang

    Pages 361-367

17. Back Matter

    Pages 369-372

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Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.

...the most comprehensive treatment available on the topic of electroporation of microorganisms...will be a useful resource for scientists working on diverse aspects of microbial genetics.-Four Stars, Doody's Health Sciences Book Review Journal

• Department of Cancer Biology, Harvard University School of Public Health, Boston

  Jac A. Nickoloff

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