Electroporation Protocols for Microorganisms

  • Jac A. Nickoloff

Part of the Methods in Molecular Biology™ book series (MIMB, volume 47)

Table of contents

  1. Front Matter
    Pages i-xix
  2. James C. Weaver
    Pages 1-26
  3. Gunter A. Hofmann
    Pages 27-45
  4. Christian E. Gruber
    Pages 67-79
  5. Herbert Weber, Hermann Berg
    Pages 93-104
  6. Elizabeth M. Miller, Jac A. Nickoloff
    Pages 105-113
  7. Kenneth E. Sanderson, P. Ronald MacLachlan, Andrew Hessel
    Pages 115-123
  8. Jonathan J. Dennis, Pamela A. Sokol
    Pages 125-133
  9. Teresa J. White, Carlos F. Gonzalez
    Pages 135-141
  10. John R. McQuiston, Gerhardt G. Schurig, Nammalwar Sriranganathan, Stephen M. Boyle
    Pages 143-148
  11. Gerald S. Baron, Svetlana V. Myltseva, Francis E. Nano
    Pages 149-154
  12. Hajime Hamashima, Makoto Iwasaki, Taketoshi Arai
    Pages 155-160
  13. Jhy-Jhu Lin
    Pages 171-178
  14. Ellyn D. Segal
    Pages 179-184
  15. Robert E. McLaughlin, Joseph J. Ferretti
    Pages 185-193
  16. Helge Holo, Ingolf F. Nes
    Pages 195-199

About this book

Introduction

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.

Editors and affiliations

  • Jac A. Nickoloff
    • 1
  1. 1.Department of Cancer BiologyHarvard University School of Public HealthBoston

Bibliographic information

  • DOI https://doi.org/10.1385/0896033104
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-310-8
  • Online ISBN 978-1-59259-534-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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