Protein Stability and Folding

Theory and Practice

  • Bret A. Shirley

Part of the Methods in Molecular Biology™ book series (MIMB, volume 40)

Table of contents

  1. Front Matter
    Pages i-x
  2. David B. Volkin, Henryk Mach, C. Russell Middaugh
    Pages 35-63
  3. Catherine A. Royer
    Pages 65-89
  4. Henryk Mach, David B. Volkin, Carl J. Burke, C. Russell Middaugh
    Pages 91-114
  5. Kunihiro Kuwajima
    Pages 115-135
  6. C. Russell Middaugh, Henryk Mach, James A. Ryan, Gautam Sanyal, David B. Volkin
    Pages 137-156
  7. Ernesto Freire
    Pages 191-218
  8. Nigel Darby, Thomas E. Creighton
    Pages 219-252
  9. Serge N. Timasheff
    Pages 253-269
  10. J. Martin Scholtz, Andrew D. Robertson
    Pages 291-311
  11. Thomas Kiefhaber
    Pages 313-341
  12. Anthony L. Fink
    Pages 343-360
  13. Back Matter
    Pages 369-377

About this book


The intent of this work is to bring together in a single volume the techniques that are most widely used in the study of protein stability and protein folding. Over the last decade our understanding of how p- teins fold and what makes the folded conformation stable has advanced rapidly. The development of recombinant DNA techniques has made possible the production of large quantities of virtually any protein, as well as the production of proteins with altered amino acid sequence. Improvements in instrumentation, and the development and refinement of new techniques for studying these recombinant proteins, has been central to the progress made in this field. To give the reader adequate background information about the s- ject, the first two chapters of this book review two different, yet related, aspects of protein stability. The first chapter presents a review of our current understanding of the forces involved in determining the conf- mational stability of proteins as well as their three-dimensional folds. The second chapter deals with the chemical stability of proteins and the pathways by which their covalent structure can degrade. The remainder of the book is devoted to techniques used in the study of these two major areas of protein stability, as well as several areas of active research. Although some techniques, such as X-ray crystallography and mass spectroscopy, are used in the study of protein stability, they are beyond the scope of this book and will not be covered extensively.

Editors and affiliations

  • Bret A. Shirley
    • 1
  1. 1.Boehringer Ingelheim Pharmaceuticals, Inc.Ridgefield

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-301-6
  • Online ISBN 978-1-59259-527-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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