Cryopreservation and Freeze-Drying Protocols

  • John G. Day
  • Michael W. Pennington

Part of the Methods in Molecular Biology™ book series (MIMB, volume 38)

Table of contents

  1. Front Matter
    Pages i-x
  2. Mark R. McLellan, John G. Day
    Pages 1-5
  3. Ernest A.
    Pages 7-20
  4. Sugio Kawamura, Yukie Murakami, Yukie Miyamoto, Kazuo Kimura
    Pages 31-37
  5. Chris J. Bond
    Pages 39-47
  6. Jacqueline A. Kolkowski, David Smith
    Pages 49-61
  7. Dirk K, Jürgen M.
    Pages 71-80
  8. John G. Day, Mitzi M. DeVille
    Pages 81-89
  9. Poula J. Reinhoud, Atsuko Uragami, Akira Sakai, Frank Van Iren
    Pages 113-120
  10. Erica E. Benson
    Pages 121-132
  11. Hugh W. Pritchard
    Pages 133-144
  12. Krishen J. Rana
    Pages 151-165
  13. Graham J. Wishart
    Pages 167-177
  14. Christopher B. Morris
    Pages 179-187
  15. Jocelyn E. Hunter
    Pages 199-209
  16. Magosaburo Kasai
    Pages 211-219
  17. Jocelyn E. Hunter
    Pages 221-234
  18. Michael J. G. Thomas, Susan H. Bell
    Pages 235-250
  19. Back Matter
    Pages 251-254

About this book


The storage of biological material for regular or future use is a fundamental requirement in many biological and medical sciences. Cryopreservation and freeze-drying are the preferred techniques for achieving long-term storage, and have been applied to a diverse range of biological materials. Though the basis for many methodologies is common, laboratories frequently lack expertise with the correct storage procedures, so that many apply outdated or inappropriate protocols for storing their samples or cultures. Cryopreservation and Freeze-Drying Protocols is a compilation of the many and varied methodologies that have been developed in expert laboratories. The protocols are reproducible, robust, and in most instances have been transferred quite successfully to other laboratories. Our intended readers are those proposing to establish or improve biostorage systems in their own laboratories or units, whether concerned with culture collections, animal husbandry, aquaculture, or human fertilization programs. Because the emphasis of Cryopreservation and Freeze-Drying Protocols is on methodology, it is our intention to provide readers with the tools to make practical progress without reference to other sources. Each chapter deals with an organelle, cell, or tissue type: a short int- duction on the status of its biostorage development is followed by a detailed description of the materials required and a methodological p- tocol to be followed, with explanatory notes. This is very much a first edition; we hope and trust that future editions will contain cryopreservation and freeze-drying protocols for ceils, tissues, and organs that are at present still recalcitrant to succe- ful preservation.

Editors and affiliations

  • John G. Day
    • 1
  • Michael W. Pennington
    • 2
  1. 1.The Windermere Laboratory, Institute of Freshwater EcologyCumbria
  2. 2.Acer EnvironmentalCheshireUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 1995
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-296-5
  • Online ISBN 978-1-59259-525-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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