Peptide Analysis Protocols

  • Ben M. Dunn
  • Michael W. Pennington

Part of the Methods in Molecular Biology book series (MIMB, volume 36)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Daniel M. Bollag
    Pages 1-9
  3. Daniel M. Bollag
    Pages 11-22
  4. Udo Nirenberg
    Pages 23-35
  5. Mark G. Hinds, Raymond S. Norton
    Pages 131-154
  6. J. Mark Carter
    Pages 193-206
  7. Ben M. Dunn, Paula E. Scarborough, Ruth Davenport, Wieslaw Swietnicki
    Pages 225-243
  8. Gino Van Heeke, Jay S. Stout, Fred W. Wagner
    Pages 245-260
  9. Gabriele Tuchscherer, Verena Steiner, Karl- Heinz Altmann, Manfred Mutter
    Pages 261-285
  10. Philip C. Andrews, Daniele M. Leonard, Wayne L. Cody, Tomi K. Sawyer
    Pages 305-328
  11. Back Matter
    Pages 329-335

About this book


As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.

Editors and affiliations

  • Ben M. Dunn
    • 1
  • Michael W. Pennington
    • 2
  1. 1.University of FloridaGainesville
  2. 2.Bachem BioscienceKing of Prussia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1994
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-274-3
  • Online ISBN 978-1-59259-523-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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