Microscopy, Optical Spectroscopy, and Macroscopic Techniques

  • Christopher Jones
  • Barbara Mulloy
  • Adrian H. Thomas

Part of the Methods in Molecular Biology book series (MIMB, volume 22)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Microscopy

    1. Front Matter
      Pages 1-1
    2. Phillip M. Williams, Mana S. Cheema, Martyn C. Davies, David E. Jackson, Saul J.B. Tendler
      Pages 25-37
  3. Scattering And SedimentatioN

  4. Calorimetric Methods

    1. Front Matter
      Pages 109-109
    2. Alan Cooper, Christopher M. Johnson
      Pages 109-124
    3. Alan Cooper, Christopher M. Johnson
      Pages 125-136
    4. Alan Cooper, Christopher M. Johnson
      Pages 137-150
  5. Optical Spectroscopy

    1. Front Matter
      Pages 151-151
    2. Alex F. Drake
      Pages 151-171
    3. Paul G. Varley
      Pages 203-218
    4. Alex F. Drake
      Pages 219-244
  6. Back Matter
    Pages 245-251

About this book


This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec­ troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand­ ing for the biochemist or biologist who needs to collaborate with spe­ cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol­ vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc­ ture using circular dichroism and Fourier-transform infrared spec­ troscopy.

Editors and affiliations

  • Christopher Jones
    • 1
  • Barbara Mulloy
    • 1
  • Adrian H. Thomas
    • 1
  1. 1.National Institute for Biological Standards and ControlPotters BarUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1994
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-232-3
  • Online ISBN 978-1-59259-509-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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