Immunochemical Protocols

1. Quantitative and Qualitative Immunoelectrophoresis

   • Anne Laine

   Pages 195-200

2. Rocket Immunoelectrophoresis Technique or Electroimmunodiffusion

   • Anne Laine

   Pages 201-205

3. Crossed Immunoelectrophoresis

   • Anne Laine

   Pages 207-213

4. Crossed Immunoaffinoelectrophoresis

   • Anne Laine

   Pages 215-219

5. Immunodetection of Proteins by Western Blotting

   • Colin J. Henderson, C. Roland Wolf

   Pages 221-233

6. Erasable Western Blots

   • Scott H. Kaufmann, Joel H. Shaper

   Pages 235-246

7. Colloidal Gold Staining and Immunoprobing on the Same Western Blot

   • Denise Egger, Kurt Bienz

   Pages 247-253

8. Colloidal Gold Staining and Immunodetection in 2-D Protein Mapping

   • Anthony H. V. Schapira

   Pages 255-259

9. Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen

   • Boguslaw Szewczyk, Donald F. Summers

   Pages 261-266

10. Competitive ELISA

    • Kitti Makarananda, Gordon E. Neal

    Pages 267-272

11. Twin-Site ELISAs for fos and myc Oncoproteins Using the AMPAK System

    • John P. Moore, David L. Bates

    Pages 273-281

12. Preparation of Cytotoxic Antibody-Toxin Conjugates

    • Alan J. Cumber, Edward J. Wawrzynczak

    Pages 283-293

13. Immunoaffinity Purification and Quantification of Antibody-Toxin Conjugates

    • Edward J. Wawrzynczak, Alan J. Cumber

    Pages 295-305

14. An Immuno-Slot-Blot Assay for Detection and Quantitation of Alkyldeoxyguanosines in DNA

    • Barbara I. Ludeke

    Pages 307-319

15. Production of Monoclonal Antibodies for the Detection of Chemically Modified DNA

    • Michael J. Tilby

    Pages 321-328

16. Sensitive Competitive Enzyme-Linked Immunoassay for Quantitation of Modified Bases in DNA

    • Michael J. Tilby

    Pages 329-335

17. An Immunochemical Assay for Detecting Transition of B-DNA to Z-DNA

    • T. J. Thomas

    Pages 337-345

18. Cell Sorting Using Immunomagnetic Beads

    • Eddie C. Y. Wang, Leszek K. Borysiewicz, Anthony P. Weetman

    Pages 347-357

19. Cell Preparation for Flow Cytometry

    • Michael G. Ormerod

    Pages 359-362

20. Preparation of Rat Lung Cells for Flow Cytometry

    • Janet Martin, Ian N. H. White

    Pages 363-368

Molecular biologists can no longer afford to be experts in only one par­ ticular area. They need to be equally competent at handling DNA, RNA, and proteins, moving from one area to another as required by the problem. Ad­ vances in the field have been greatly helped by numerous applications of antibody-antigen interactions, so that a basic knowledge of immunology has now become useful. The emphasis of Immunochemical Protocols is not on the production of antibodies, since many people will beg or buy these, but rather on the enor­ mous range of applications to which these powerful reagents can be put. For completeness, however, methods are included for the production of both polyclonal and monoclonal antibodies. Techniques that may be helpful in obtaining antibodies to elusive antigens and to synthetic peptides are also provided. Both qualitative and quantitative methods are described for local­ izing, characterizing, and quantifying various antigens. These range from the use of antibodies in screening DNA libraries to their application in EUSA assays for quantifying adducts in alkylated DNA Each method is described by an author who has regularly used the tech­ in his or her own laboratory. Not all the techniques necessarily repre­ nique sent the state-of-the-art-they are, however, all dependable methods that regularly achieve the desired result. Each chapter starts with a brief description of the basic theory behind the technique being described. The main aim of this book, however, is to outline the practical steps necessary for carrying out the method successfully.

Beyond question...the book will be used in many laboratories....[A] fine addition to the...library of publications on immunological methods.-Molecular Immunology

• Medical Research Council Laboratories, Carshalton, UK

  Margaret M. Manson

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