Immunochemical Protocols

  • Margaret M. Manson

Part of the Methods in Molecular Biology book series (MIMB, volume 10)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Jonathan A. Green, Margaret M. Manson
    Pages 1-5
  3. Monique Diano, André Le Bivic, Michel Hirn
    Pages 13-22
  4. David C. Hancock, Gerard I. Evan
    Pages 23-32
  5. Michael G. Baines, Robin Thorpe
    Pages 79-104
  6. Sara E. Mole
    Pages 105-116
  7. Michael G. Ormerod, Susanne F. Imrie
    Pages 117-124
  8. Jonathan A. Green, Richard E. Edwards, Margaret M. Manson
    Pages 131-135
  9. Edward A. Bayer, Meir Wilchek
    Pages 137-142
  10. Edward A. Bayer, Meir Wilchek
    Pages 143-148
  11. Edward A. Bayer, Meir Wilchek
    Pages 149-162
  12. Julian E. Beesley
    Pages 163-168
  13. Julian E. Beesley
    Pages 169-176
  14. Julian E. Beesley
    Pages 177-185

About this book

Introduction

Molecular biologists can no longer afford to be experts in only one par­ ticular area. They need to be equally competent at handling DNA, RNA, and proteins, moving from one area to another as required by the problem. Ad­ vances in the field have been greatly helped by numerous applications of antibody-antigen interactions, so that a basic knowledge of immunology has now become useful. The emphasis of Immunochemical Protocols is not on the production of antibodies, since many people will beg or buy these, but rather on the enor­ mous range of applications to which these powerful reagents can be put. For completeness, however, methods are included for the production of both polyclonal and monoclonal antibodies. Techniques that may be helpful in obtaining antibodies to elusive antigens and to synthetic peptides are also provided. Both qualitative and quantitative methods are described for local­ izing, characterizing, and quantifying various antigens. These range from the use of antibodies in screening DNA libraries to their application in EUSA assays for quantifying adducts in alkylated DNA Each method is described by an author who has regularly used the tech­ in his or her own laboratory. Not all the techniques necessarily repre­ nique sent the state-of-the-art-they are, however, all dependable methods that regularly achieve the desired result. Each chapter starts with a brief description of the basic theory behind the technique being described. The main aim of this book, however, is to outline the practical steps necessary for carrying out the method successfully.

Editors and affiliations

  • Margaret M. Manson
    • 1
  1. 1.Medical Research Council LaboratoriesCarshaltonUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896032043
  • Copyright Information Humana Press 1992
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-204-0
  • Online ISBN 978-1-59259-497-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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