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The Hepatocyte Review

  • Editors
  • Michael N. Berry
  • Anthony M. Edwards

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Methods and Biology

    1. Michael N. Berry
      Pages 1-9
    2. María José Gómez-Lechón, José V. Castell
      Pages 11-15
    3. Hans G. Koebe, Sascha A. Pahernik, Friedrich W. Schildberg
      Pages 17-26
    4. Michael G. Clark, Michelle A. Vincent
      Pages 27-36
    5. Nazzareno Ballatori, Gregory C. Connolly, James L. Boyer
      Pages 37-47
    6. Thomas Braunbeck, Helmut Segner
      Pages 49-71
    7. Anthony M. Edwards, George K. Michalopoulos
      Pages 73-96
    8. Augustinus Bader, Erich Knop, Axel Haverich
      Pages 97-115
    9. Hans G. Koebe, Sascha A. Pahernik, Friedrich W. Schildberg
      Pages 117-123
    10. André Guillouzo, Claire Guyomard, Alain Fautrel, Christophe Chesné
      Pages 125-145
    11. Peter E. Declercq, Myriam I. Baes
      Pages 169-180
    12. Toshihiro Mitaka, Fumihiko Sato
      Pages 195-208
  3. Reviews of Hepatocyte Biology

    1. Thoralf Christoffersen, G. Hege Thoresen, Olav F. Dajani, Øyvind Melien, Tormod Guren, Magne Refsnes et al.
      Pages 209-246
    2. María José Gómez-Lechón, Isabel Guillén, M. J. Teresa Donato, Xavier Ponsoda, Ramiro Jover, José V. Castell
      Pages 247-261
    3. Gennady Ilyin, Claude Rescan, Mickaël Rialland, Pascal Loyer, Georges Baffet, Christiane Guguen-Guillouzo
      Pages 263-280
    4. Christopher A. Bombeck, Jianrong Li, Young-Myeong Kim, Timothy R. Billiar
      Pages 301-315
    5. John W. Phillips, Marlene B. Grivell, Anthony R. Grivell, Michael N. Berry
      Pages 343-363
  4. Applications: Pharmacology and Toxicology

  5. Therapeutic Applications

    1. Robert A. Fisher, Stephen C. Strom
      Pages 475-501
    2. Steven E. Raper, A. David Moscioni
      Pages 503-520
    3. Mark D. Smith, M. Helen Grant, Rod B. Cousins, John D. S. Gaylor
      Pages 521-541
    4. Jacek Rozga, Achilles A. Demetriou
      Pages 543-559
    5. Benedikt Busse, Jörg Gerlach
      Pages 561-573
    6. Robert A. F. M. Chamuleau, Leonard M. Flendrig
      Pages 575-583
  6. Back Matter
    Pages 597-605

About this book

Introduction

It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.

Keywords

animals apoptosis biology cell cell cycle cytochrome P450 development gene gene therapy membrane metabolism mitochondria regulation system

Bibliographic information

  • DOI https://doi.org/10.1007/978-94-017-3345-8
  • Copyright Information Springer Science+Business Media B.V. 2000
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-90-481-5402-9
  • Online ISBN 978-94-017-3345-8
  • Buy this book on publisher's site
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