In Vitro Culture of Higher Plants

  • R. L. M. Pierik

Table of contents

  1. Front Matter
    Pages N1-V
  2. R. L. M. Pierik
    Pages 3-20
  3. R. L. M. Pierik
    Pages 21-28
  4. R. L. M. Pierik
    Pages 29-30
  5. R. L. M. Pierik
    Pages 31-43
  6. R. L. M. Pierik
    Pages 45-82
  7. R. L. M. Pierik
    Pages 83-85
  8. R. L. M. Pierik
    Pages 87-88
  9. R. L. M. Pierik
    Pages 89-94
  10. R. L. M. Pierik
    Pages 95-99
  11. R. L. M. Pierik
    Pages 101-105
  12. R. L. M. Pierik
    Pages 127-132
  13. R. L. M. Pierik
    Pages 133-138
  14. R. L. M. Pierik
    Pages 139-148
  15. R. L. M. Pierik
    Pages 149-158
  16. R. L. M. Pierik
    Pages 159-167
  17. R. L. M. Pierik
    Pages 169-181
  18. R. L. M. Pierik
    Pages 183-230

About this book

Introduction

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects.
Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

Keywords

Embryo development fertilization growth orchid orchid seeds phytopathology plant plant breeding plants soil

Authors and affiliations

  • R. L. M. Pierik
    • 1
  1. 1.Department of HorticultureWageningen Agricultural UniversityThe Netherlands

Bibliographic information

  • DOI https://doi.org/10.1007/978-94-011-5750-6
  • Copyright Information Springer Science+Business Media Dordrecht 1997
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-7923-4527-5
  • Online ISBN 978-94-011-5750-6
  • About this book
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