Molecular Tools for Screening Biodiversity

1. Front Matter

   Pages i-xxiv

   PDF

2. DNA Extraction

   1. Front Matter

      Pages 1-1

      PDF

   2. DNA Extraction: widely applicable methods

      1. Isolation of Animal Cellular Total DNA

         • De-Xing Zhang, Godfrey M. Hewitt

         Pages 5-9

      2. Plant Total DNA Extraction

         • Julia Rueda, Rosario Linacero, Ana M. Vázquez

         Pages 10-14

      3. Chloroplast DNA Extraction Procedure for Species High in Phenolics and Polysaccharides

         • Roselyne Lumaret, Henri Michaud, Jean-Philippe Ripoll, Lamjed Toumi

         Pages 15-17

      4. Quantification of DNA

         • Rosario Linacero, Julia Rueda, Ana M. Vázquez

         Pages 18-21

      5. Miniprep Procedures for the Isolation of Plant DNA

         • Keith J. Edwards

         Pages 22-24

   3. DNA Extraction: methods for difficult species/tissues

      1. Special DNA Extraction Methods for Some Animal Species

         • De-Xing Zhang, Godfrey M. Hewitt

         Pages 27-31

      2. Methods for Difficult Plant Species/Tissues

         • Birgit Ziegenhagen, Florian Scholz

         Pages 32-35

      3. DNA Maxipreparations: A Comparison of Protocols for Rapid Extraction of Good Quality DNA from Recalcitrant Species

         • Birgit Ziegenhagen, Florian Scholz

         Pages 36-37

   4. Preserved specimens

      1. Isolation of DNA from Preserved Specimens

         • De-Xing Zhang, Godfrey M. Hewitt

         Pages 41-45

      2. Field Collection: Animals

         • De-Xing Zhang, Godfrey M. Hewitt

         Pages 46-48

      3. Field Collection: Plants

         • Roger Hyam

         Pages 49-50

   5. DNA Extraction using anion-exchange chromatography and silica-gel based membranes

      1. DNA Extraction Using Anion-Exchange Chromatography and Silica-Gel Based Membranes

         • Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian

         Pages 53-54

      2. Isolation of Nucleic Acids Using Anion-Exchange Chromatography: QIAGEN-tip Based Methods

         • Angela Karp, Peter G. Isaac, David S. Ingram

         Pages 54-59

      3. Isolation of Nucleic Acids Using Silica-Gel Based Membranes: Methods Based on the Use of QIAamp Spin Columns

         • Angela Karp, Peter G. Isaac, David S. Ingram

         Pages 59-63

      4. Isolation of Total RNA from Animal Tissue Using Anion-Exchange Chromatography (QIAGEN-tips)

         • Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian

         Pages 64-66

      5. Isolation of Total RNA Using Silica-Gel Based Membranes

         • Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian

         Pages 67-70

3. Basic Screening Methods

   1. Front Matter

      Pages 71-71

      PDF

   2. Isozymes

      1. Isozymes

         • Gerhard Müller-Starck

         Pages 75-81

Mark Chase There are many literature resources available to molecular biologists wishing to assess genetic variation, but the myriad of techniques and approaches potentially available to the plant breeder and the evolutionary biologist is truly bewildering, and most have never been evaluated side-by-side on the same sets of samples. Additionally, it is often not recognized that tools that are useful for breeders can often be adapted for use in evolutionary studies and vice versa, but this is generally the case. The borderline between population genetics and phylogenetics is vague and difficult to assess, and a combination of both types of tools is best when it is not clear with which area one is dealing. Furthermore, it is not now appropriate to use just one type of marker in any kind of study; most markers have the potential to misinform under certain conditions, so it is always wise to incorporate at least two different types of assessments into any project. This volume is designed to facilitate this sort of multiple approach and provides comparative data on most currently available methods so that researchers can more intelligently select those appropriate to their area of interest, regardless of whether it is in the realm of breeding or evolutionary biology.

• biodiversity
• classification
• evolution
• morphology
• phylogeny
• tissue

• IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, UK

  Angela Karp

• Agrogene SA, Moissy Cramayel, France

  Peter G. Isaac

• Regius Keeper of the Royal Botanic Garden Edinburgh, UK

  David S. Ingram

Policies and ethics