Cells and Culture

1. Applied Integrative Physiology

   1. Influence of Glucose and Glutamine Concentration on Metabolism of rCHO Cells

      • Hiroshi Matsuoka, Jun-ya Watanabe, Toshiya Takeda

      Pages 107-111

2. Speed and Intensification in Bioprocess Development

   1. Front Matter

      Pages 113-113

      PDF

   2. Delivery of Biomolecules with Non-Viral Vectors

      • Stéphanie Biri, Fabrice Stock, Abdennaji Adib, Patrick Erbacher

      Pages 115-119

   3. Circumventing the “Pay Now or Pay Later” Dilemma: Strategies for Achieving Process Development with Speed and Long-Term Potential

      • Carole Heath, Pranhitha Reddy, Martin Allen, Ginny Price, Victor Fung

      Pages 121-128

   4. Recombinant Human Antibody Therapeutics: Supply Strategies for Early and Clinical Development from CHO Cells

      • Ray Field, Lekan Daramola, Jonathan Dempsey, Diane Hatton

      Pages 129-136

   5. Automated Screening of High Producer HEK293F Clones and Analysis of Post-Translational Modifications of Secreted Proteins

      • Peter Söhlemann, Claudia Kollert, Jessica Thiems, Elisabeth Casademunt, Carola Schröder

      Pages 137-144

   6. Transcriptomic and Proteomic Analysis of Antibody Producing NS0 Cells Cultivated at Different Cell Densities in Perfusion Culture

      • Britta Krampe, Halina Swiderek, Mohamed Al-Rubeai

      Pages 145-149

   7. New Disposable Fixed-Bed Bioreactor for Cell Culture and Virus Production Based on a Proprietary Agitation and Aeration System

      • J.-C. Drugmand, N. Havelange, S. Osumba, F. Bosco, F. Debras, F. Collignon et al.

      Pages 151-155

   8. Transcriptomic Analysis of Antibody Producing NS0 Cell Line Under Hypothermic and Hypoxic Conditions

      • Halina Swiderek, Anna Logan, Mohamed Al-Rubeai

      Pages 157-160

   9. Semi-Continuous Cultures as a Tool for Cell Line Characterization During Process Development

      • S. Victores, A. Castillo, E. Faife, Y. Rabasa, Y. Alvarez, L. Rojas et al.

      Pages 161-164

   10. Behaviour of GS-CHO Cell Lines in a Selection Strategy

       • A. J. Porter, A.J. Dickson, L.M. Barnes, A.J. Racher

       Pages 165-169

   11. 3D Cultures: Effect on the Hepatocytes Functionality

       • Joana Miranda, Manuel J.T. Carrondo, Paula M. Alves

       Pages 171-176

   12. Differential Protein Expression Induced by c-Myc Over-Expression: Proteomic Analysis of a CHO Cell Line with Increased Proliferation Capacity

       • Darrin Kuystermans, Mohamed Al-Rubeai

       Pages 177-181

   13. Development of Pilot-Scale Orbital Shake Bioreactors: Ideal for Cost-Effective and Efficient Transient Gene Expression

       • Matthieu Stettler, Xiaowei Zhang, Tibor Anderlei, Maria De Jesus, Pascal Lefebvre, David L. Hacker et al.

       Pages 183-185

   14. Helical Tracks in Shaken Cylindrical Bioreactors Improve Oxygen Transfer and Increase Maximum Cell Density Obtainable for Suspension Cultures of Mammalian Cells

       • Xiaowei Zhang, Matthieu Stettler, Oscar Reif, Andreas Kocourek, Maria De Jesus, David L. Hacker et al.

       Pages 187-191

   15. Dynamic Optimisation of CHO-IFNγ Cell Culture Fed-Batch Time-Profile

       • Carolyn M. Lam, Danny C.F. Wong, Miranda G.S. Yap, Efstratios N. Pistikopoulos, Athanasios Mantalaris

       Pages 193-199

   16. Long-Term 3D-Culture of HEP G2 Cell Line on Macroporous Ceramic Carriers

       • Oscar Platas, Vivien Lutz, Richard Getto, Ralf Pörtner

       Pages 201-204

   17. An Integrated Production Process for Human Growth Hormone

       • T. Nottorf, H. Büntemeyer, S. Siwiora-Brenke, D. Lütkemeyer, A. Loa, T. Noll et al.

       Pages 205-208

   18. Efficient Production of Human Monoclonal Antibodies by an Improved Fructose-Based Human Cell Culture

       • Yuichi Inoue, Aiko Inoue, Hiroharu Kawahara

       Pages 209-212

   19. Coupling Between Cell Kinetics and CFD to Establish Physio-Hydrodynamic Correlations in Various Stirred Culture Systems

       • N. Barbouche, E. Olmos, E. Guedon, A. Marc

       Pages 213-217

Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.

• Proteomics
• bioinformatics
• biotechnology
• cell
• cell culture
• glycoprotein
• microarray
• physiology
• protein
• proteins

• Technische Fak., AG Zellkulturtechnik, Universität Bielefeld, Bielefeld, Germany

  Thomas Noll

Policies and ethics