Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification

  • Jianpeng Wang

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Jianpeng Wang
    Pages 1-14
  3. Jianpeng Wang
    Pages 15-25
  4. Jianpeng Wang
    Pages 27-45
  5. Jianpeng Wang
    Pages 71-93
  6. Jianpeng Wang
    Pages 95-96

About this book

Introduction

This thesis focuses on the study of interactions between protein and peptides and their potential applications in cell imaging and nanoparticle surface modification. Drawing inspiration from naturally occurring coiled-coil binding pairs, it proposes a novel covalent peptide tag and probe system, based on the concept of “affinity guided covalent conjugation.” This newly established methodology provides complementary resolution to protein labeling, imaging and trafficking. By systematically investigating the coordination interaction between protein and quantum dots using various engineered protein ligands, this thesis proposes a general rule for protein self-assembly on the surface of quantum dots and reports a revolutionized nanobelt protein in accordance with this rule. It is an extraordinary example of interdisciplinary research, providing answers to real-life biological problems from a chemistry perspective.

Keywords

Coiled-coil interaction Covalent protein conjugation Peptide tag Protein folding Protein self-assembly Protein-peptide interactions Antifungal peptides Protein ligands engineering Cell labeling Protein-nanoparticle assemblies

Authors and affiliations

  • Jianpeng Wang
    • 1
  1. 1.Department of ChemistryThe Chinese University of Hong KongHong KongChina

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-53399-4
  • Copyright Information Springer-Verlag Berlin Heidelberg 2016
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-662-53397-0
  • Online ISBN 978-3-662-53399-4
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • About this book
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