Hydrocarbon and Lipid Microbiology Protocols

Field Studies

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Roger C. Prince
    Pages 1-25
  3. Thomas L. Kieft
    Pages 45-64
  4. Nicolas Tsesmetzis, Michael J. Maguire, Ian M. Head, Bart P. Lomans
    Pages 65-109
  5. Christina M. Ridley, Gerrit Voordouw, Lisa M. Gieg
    Pages 111-131
  6. Michael Cunliffe, Oliver Wurl
    Pages 255-261
  7. Ryan Sibert, Sarah Harrison, Samantha B. Joye
    Pages 263-276
  8. Robert Duran, Cristiana Cravo-Laureau
    Pages 305-317
  9. Estelle Couradeau, Karim Benzerara, David Moreira, Purificación López-García
    Pages 319-341

About this book

Introduction

This Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer, mud flats, microbialites, and deep-sea fauna. A variety of downstream analytical procedures are described, including: nucleic-acid extraction and preparation for high-throughput sequencing, fluorescence in-situ hybridisation, and cultivation of aerobic and anaerobic hydrocarbon-degrading microbes. Though most chapters focus on hydrocarbon-rich environments, many of the approaches used are generic, and as such will be of value to researchers embarking on studies of microbes and their processes in the field.

Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.



Keywords

Analysis of Marine Systems Biodegradation Bioremediation Ecotoxicology Groundwater/Freshwater Terrestrial Systems

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.University of EssexSchool of Biological Sciences University of EssexColchesterUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Department of BiologyUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-53118-1
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-662-53116-7
  • Online ISBN 978-3-662-53118-1
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • About this book

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