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Hydrocarbon and Lipid Microbiology Protocols

Microbial Quantitation, Community Profiling and Array Approaches

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Michael Lappé, Jens Kallmeyer
    Pages 7-16
  3. Stefan J. Green, Mary Beth Leigh, Josh D. Neufeld
    Pages 77-99
  4. Christoph C. Tebbe, Anja B. Dohrmann, Michael Hemkemeyer, Astrid Näther
    Pages 101-126
  5. Yolanda Blanco, Mercedes Moreno-Paz, Jacobo Aguirre, Victor Parro
    Pages 207-224
  6. Julia Johnke, Antonis Chatzinotas
    Pages 225-252

About this book

Introduction

This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Keywords

Microbial communities Oily sediments Hydrocarbon-contaminated environments Hydrocarbon-degrading bacterial communities Microbial biomass

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.University of Essex Dept. Biological SciencesColchesterUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Dept. BiologiaUniv de les Illes BalearsPalma de MallorcaSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-52778-8
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-662-52776-4
  • Online ISBN 978-3-662-52778-8
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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