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Electron Microscopy of Axon Degeneration: A Valuable Tool in Experimental Neuroanatomy

  • John F. Alksne
  • Theodor W. Blackstad
  • Fred Walberg
  • Lowell E. WhiteJr.
Conference proceedings

Table of contents

  1. Front Matter
    Pages 1-5
  2. John F. Alksne, Theodor W. Blackstad, Fred Walberg, Lowell E. White Jr.
    Pages 6-7
  3. John F. Alksne, Theodor W. Blackstad, Fred Walberg, Lowell E. White Jr.
    Pages 7-13
  4. John F. Alksne, Theodor W. Blackstad, Fred Walberg, Lowell E. White Jr.
    Pages 13-25
  5. John F. Alksne, Theodor W. Blackstad, Fred Walberg, Lowell E. White Jr.
    Pages 25-29
  6. John F. Alksne, Theodor W. Blackstad, Fred Walberg, Lowell E. White Jr.
    Pages 29-29
  7. Back Matter
    Pages 29-33

About these proceedings

Introduction

Experimental methods for the mapping of nervous pathways are based partlyon the study of retrograde processes in the perikaryon, partlyon the demonstration of degenerative processes along the peripheral part of a transected axon. For this purpose, the Marchi method by which a selective staining of degenerating myelin is obtained has been extensively used. However, when this method is used the non-myelinated terminals of the transected axons are not stained. The introduction, about two decades ago, of silver impregnation as a means of tracing degenerating axons (especially the Glees and Nauta methods) by which also terminal boutons can be demonstrated, led therefore to revolutionary progress in the investigation of interneuronal connections. Notwithstanding, there are weH known difficulties involved in this kind of research. The capriciousness of the silver methods not seldom results in failure of impregnation with loss of valuable experimental animals. But even when well impregnated sections are used, other fundamental difficulties exist. One of the major problems is to prove beyond doubt that the impregnated structures are degenerating boutons and not merely fragments of non-terminal fibres passing the area under examination. Furthermore, only on occasion will silver impregnation permit one to accurately define the specific part of the receiving neuron on which the impregnated fibres end, i. e. , whether the bouton makes contact with soma, dendrite or spine.

Keywords

Axon anatomy animals electron microscopy microscopy myelin neuroanatomy

Authors and affiliations

  • John F. Alksne
    • 1
  • Theodor W. Blackstad
    • 2
  • Fred Walberg
    • 2
  • Lowell E. WhiteJr.
    • 3
  1. 1.Harbor General HospitalTorranceUSA
  2. 2.Anatomisk InstituttUniversitetet i OsloOslo 1Norge
  3. 3.The Medical SchoolUniversity of WashingtonSeattle 5USA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-662-30450-1
  • Copyright Information Springer-Verlag Berlin Heidelberg 1966
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-03493-3
  • Online ISBN 978-3-662-30450-1
  • Series Print ISSN 0301-5556
  • Series Online ISSN 2192-7065
  • Buy this book on publisher's site
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