Preparative Chromatography Techniques

1. Front Matter

   Pages i-ix

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2. Introduction

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 1-1

3. Sample Preparation and Purification

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 2-5

4. Planar Chromatography

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 6-18

5. Special Column Chromatography

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 19-26

6. Preparative Pressure Liquid Chromatography

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 27-79

7. Counter-Current Chromatography

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 80-126

8. Combination of Methods

   • Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

   Pages 127-135

9. Back Matter

   Pages 137-142

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Over the last few years several new instrumental techniques have been introduced for chromatographie separations. In addition, rapid developments in existing methods, such as preparative HPLC, have taken place. Despite these advances, how­ ever, a handbook covering the various preparative aspects ofthe new separation tech­ niques does not exist. This book is an attempt to fill the gap and to present a compila­ tion of modern separation techniques that will be useful for researchers faced with day-to-day preparative problems. Numerous examples of separations have been selected in order to show the possibilities (and also the limits) of each technique treated. These are often either applications from our own laboratory or else they reflect the approach we have been following for the isolation of natural products from plant sourees. Owing to the large number of published papers and the diversity of secondary plant constituents. an exhaustive survey of the literature has not been undertaken. We hope, however, that the examples selected will suggest to the reader which technique(s) and which conditions to choose for a particular isolation problem in the field of natural products. For invaluable help in the preparation of the manuscript for this book, we would like to thank Corinne Appolonia and Christine Marston. Lausanne, November 1985 K. Hostettmann M. Hostettmann A . . Marston Foreword Although not many people realize this, isolation and purification of biologically active materials is becoming increasingly crucial.

• Chromat
• biological
• chromatography
• column chromatography
• development
• isolation
• natural product
• paper
• plant
• purification
• separation

• Institute of Pharmacognosy and Phytochemistry, School of Pharmacy, University of Lausanne, Lausanne, Switzerland

  Kurt Hostettmann, Maryse Hostettmann, Andrew Marston

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