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The Cytochemical Bioassay of Polypeptide Hormones

  • Book
  • © 1980

Overview

Authors:
  1. Joseph Chayen

    1. Division of Cellular Biology, The Mathilda and Terence Kennedy Institute of Rheumatology, London, UK

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Part of the book series: Monographs on Endocrinology (ENDOCRINOLOGY, volume 17)

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Table of contents (15 chapters)

  1. Front Matter

    Pages I-XIV
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  2. Nature of Cytochemistry

    • Joseph Chayen
    Pages 1-11

  3. Basic Concepts of Hormone Assays

    • Joseph Chayen
    Pages 12-19

  4. Introduction to Cytochemical Bioassay

    • Joseph Chayen
    Pages 20-42

  5. Maintenance Culture

    • Joseph Chayen
    Pages 43-51

  6. Cytochemical Methods

    • Joseph Chayen
    Pages 52-68

  7. Microdensitometry

    • Joseph Chayen
    Pages 69-79

  8. The Segment Assay of the Adrenocorticotrophic Hormone

    • Joseph Chayen
    Pages 80-93

  9. The Section Assay of Adrenocorticotrophin

    • Joseph Chayen
    Pages 94-99

  10. Application of Cytochemical Bioassays of Adrenocorticotrophin

    • Joseph Chayen
    Pages 100-105

  11. The Assay of Thyroid Stimulators

    • Joseph Chayen
    Pages 106-129

  12. Luteinizing Hormone

    • Joseph Chayen
    Pages 130-139

  13. Gastrin-like Activity

    • Joseph Chayen
    Pages 140-155

  14. Parathyroid Hormone

    • Joseph Chayen
    Pages 156-165

  15. Hypophysiotrophic Hormones: Two-Step Bioassays

    • Joseph Chayen
    Pages 166-170

  16. Prospects: Uses of the Cytochemical Bioassay System

    • Joseph Chayen
    Pages 171-175

  17. Back Matter

    Pages 175-210
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Keywords

About this book

The cytochemical bioassay system was described in a short abstract in 1971, and more fully, in the cytochemical bioassay of corticotrophin, in 1972. Since then, cytochemical bioassays have been described for several polypeptide hormones, and these assays are already widely used. It is expedient that the subject should be reviewed, as it is in this monograph, by one writer who has had the good fortune to have taken part in the growth of cytochemistry from its early origins to its present position as the basis of possibly the most sensitive bioassay system currently available. However, it should be noted that major contributions have been made by many, both to the development of the subject and to the establishment of the bioassays. The object of this preface is to try to give some perspective to the growth of this subject and to record that the cytochemical bioassay system has been fostered by many outstanding scientists in an atmosphere of remarkable goodwill. To begin with, there could have been no cytochemical bioassays until cytochem­ istry had been converted from its rather unsure origins into a precise and quantitative form of cellular biochemistry. This was done with skill and enthusiastic dedication by my colleagues, Dr. Lucille Bitensky, Dr. F. P. Altman, Dr. R. G. L. W. Poulter and Mr. A. A. Silcox, first at the Royal College of Butcher, Dr.

Authors and Affiliations

  • Division of Cellular Biology, The Mathilda and Terence Kennedy Institute of Rheumatology, London, UK

    Joseph Chayen

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