Hemopoietic Colonies

1. Front Matter

   Pages i-ix

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2. Historical Introduction

   • Donald Metcalf

   Pages 1-4

3. An Outline of Hemopoiesis and Current Terminology

   • Donald Metcalf

   Pages 5-11

4. Techniques for the Clonal Culture of Hemopoietic Cells in Semisolid Medium

   • Donald Metcalf

   Pages 12-35

5. General Features of Hemopoietic Colony Formation

   • Donald Metcalf

   Pages 36-56

6. Neutrophil and Macrophage Colony Formation by Normal Cells

   • Donald Metcalf

   Pages 57-98

7. Colony Formation by Myeloid Leukemic Cells

   • Donald Metcalf

   Pages 99-131

8. GM-Colony Formation in Myeloproliferative Disorders

   • Donald Metcalf

   Pages 132-139

9. Eosinophil Colony Formation

   • Donald Metcalf

   Pages 140-149

10. Megakaryocyte Colony Formation

    • Donald Metcalf

    Pages 150-159

11. Erythroid Colony Formation

    • Donald Metcalf

    Pages 160-169

12. Normal Mouse and Human Lymphocyte Colonies

    • Donald Metcalf

    Pages 170-186

13. Neoplastic Lymphoid and Plasmacytoma Colonies

    • Donald Metcalf

    Pages 187-194

14. Hemopoietic Stromal Colonies

    • Donald Metcalf

    Pages 195-199

15. General Discussion

    • Donald Metcalf

    Pages 200-205

16. Back Matter

    Pages 206-230

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The cloning of hemopoietic cells in semisolid medium began some 12 years ago, and when colonies of T = and B = lymphocytes were grown by several groups last year, the last major subclasses of hemopoietic cells had been successfully cultured in vitro. The time seemed appropriate to write a short general account of this exciting new area of hematology, emphasizing particularly the potential value of these new techniques for direct studies on patients with leukemia and allied diseases. I had hoped to refer to, or at least record, every paper published in this field, but the literature proved to be larger than I had realized, and furthermore, some areas were so active that chapters had to be rewritten over a period of less than a year. I regret that ·what follows is an incomplete account and no doubt, by the time this book becomes available, the material will be even more out of date. However, I have described the basic techniques in some detail and have tried, where possible, to explain how and why the techniques can fail. It is already possible to see patterns, perhaps even principles, emerging from this work which may well be applicable to other cell populations. These have been emphasized in the text in an attempt to make this review as creative as possible.

• cell
• diseases
• hematology
• leukemia
• lymphocytes

• Cancer Research Unit, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

  Donald Metcalf

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