Biological Membranes: Structure, Biogenesis and Dynamics

1. Front Matter

   Pages I-X

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2. Role of Phospholipids in Cell Function

   • William Dowhan

   Pages 1-21

3. Assembly of Sphingolipids into Membranes of the Yeast, Saccharomyces Cerevisiae

   • P. Hechtberger, E. Zinser, F. Paltauf, G. Daum

   Pages 23-31

4. Localization of Phospholipids in Plasma Membranes of Mammalian Cells

   • J. A. F. Op den Kamp, E. Middelkoop, R. J. Ph. Musters, J. A. Post, B. Roelofsen, A. J. Verkleij

   Pages 33-41

5. Some Aspects of Intracellular Lipid Traffic

   • Vytas A. Bankaitis

   Pages 43-51

6. Intracellular Localization and Mechanisms of Regulation of Phosphatidylinositol Transfer Protein in Swiss Mouse 3T3 Cells

   • G. T. Snoek, K. W. A. Wirtz

   Pages 53-59

7. Phosphatidylinositol Transfer Protein Function in Yeast

   • Vytas A. Bankaitis

   Pages 61-70

8. Conversion of the amphiphilic 115 kDa Form of Glycosyl-Phosphatidylinositol-specific Phospholipase D to an active, hydrophilic 47 kDa Form

   • Marius C. Hoener, Jian Liao, Urs Brodbeck

   Pages 71-78

9. Magnetic Resonance Studies of Protein-Lipid Interactions

   • Anthony Watts

   Pages 79-92

10. Lipid-Protein Interactions with the Hydrophobic SP-B and SP-C Lung Surfactant Proteins in Dipalmitoylphosphatidylcholine Bilayers

    • Jesús Pérez-Gil, Cristina Casals, Derek Marsh

    Pages 93-100

11. Protein-Membrane Interactions in the Complex Biological Milieu

    • A. Chonn, S. C. Semple, P. R. Cullis

    Pages 101-106

12. Protein Sorting and Glycolipid-Enriched Detergent-Insoluble Complexes in Epithelial Cells

    • K. Fiedler, P. Dupree, K. Simons

    Pages 107-114

13. Structural and Functional Consequences of Acylation of a Transmembrane Peptide

    • T. C. B. Vogt, J. A. Killian, B. de Kruijff

    Pages 115-130

14. Non-Crystallographic Methods to Study Membrane Proteins

    • Anthony Watts

    Pages 131-138

15. Preferred Local Conformations of Peptides comprising the Entire Sequence of Bovine Pancreatic Trypsin Inhibitor (BPTI) and their Roles in Protein Folding

    • Johan Kemmink, Thomas E. Creighton

    Pages 139-153

16. Co-translational Modification, Stability and Turnover of Eukaryotic Proteins

    • Ralph A. Bradshaw, Jose Sy, Albert E. Stewart, Richard L. Kendall, Hubert Hondermarck, Stuart M. Arfin

    Pages 155-167

17. Structure and Mechanism of Porins

    • Georg E. Schulz

    Pages 169-179

18. The Pore-Forming Toxin Aerolysin: From the Soluble to a Transmembrane Form

    • F. Gisou van der Goot, J. Thomas Buckley, Franc Pattus

    Pages 181-190

19. Signal Sequences: Roles and Interactions by Biophysical Methods

    • Lila M. Gierasch

    Pages 191-198

20. Assembly of Integral Membrane Proteins

    • Gunnar von Heijne

    Pages 199-205

The Advanced Study Institute on "Structure, Biogenesis and Dynamics of Biological Membranes, held in Cargese from June 14-26, 1993, has been dealing with four major topics in membrane biochemistry today: lipid dynamics and lipid-protein interactions, protein translocation and insertion, intracellular traffic aud protein structure and folding. The lecturers discussed these topics starting from several disciplines, including biochemistry, cell biology, genetics, and biophysics. This wayan interdisciplinary and very inte~sting view on biological membrane systems was obtained. At first an extensive overview of -mainly biophysical -techniques which can be used to study dynamic processes in membranes was presented. Sophisticated approaches such as ESR and NMR have been applied succesfully to unravel details of specific lipid-protein interactions. x­ ray analysis provides detailed structural information of several proteins and the possible implications for protein functions. Information obtained this way is complemented by studies on mechanisms and kinetics of protein folding. The latter information is indispensable when discussing protein translocation and insertion: proces:;es in which folding and unfolding play essential roles. Extensive insight was offered in the complicated machinery of phospholipid biosynthesis. In particular, the application of sophisticated genetic techniques has allowed a better understanding of the mechanisms regulating the synthetic machinery and detailed studies on a variety of mutants, lacking one or more of the essential enzymes, have resulted in the beginning of a bL!:

• ATP
• Lipid
• biomembrane
• cell
• chaperones
• enzyme
• enzymes
• gene
• membrane
• protein
• protein folding
• proteins
• regulation
• synthesis
• translation

• Centre for Biomembranes and Lipid Enzymology, Utrecht, The Netherlands

  Jos A. F. Op den Kamp

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