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Electron Microscopy of Plant Pathogens

  • Kurt Mendgen
  • Dietrich-Eckhardt Lesemann

Table of contents

  1. Front Matter
    Pages I-XV
  2. Kurt Mendgen, Klaus Welter, Frank Scheffold, Gertrude Knauf-Beiter
    Pages 31-42
  3. Michel Wurtz
    Pages 73-86
  4. Giovanni P. Martelli
    Pages 103-117
  5. Jan W. M. Van Lent, Benedictus J. M. Verduin
    Pages 119-131
  6. Detlev Riesner, J. Harders, N. Lukacs, R. Gruner, U. J. Santore, G. Klotz et al.
    Pages 161-176
  7. Ian Brown, John Mansfield
    Pages 185-196
  8. Richard J. O’Connell, John A. Bailey
    Pages 211-222
  9. D. E. Harder, J. Chong
    Pages 235-250
  10. Richard J. Howard, Timothy M. Bourett, Margaret A. Ferrari
    Pages 251-264
  11. Paola Bonfante-Fasolo, Silvia Perotto
    Pages 265-275
  12. Burton Y. Endo
    Pages 291-305
  13. Back Matter
    Pages 333-336

About this book

Introduction

Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.

Keywords

Cryofixation Elektronenmikroskopie Gefrierfixierung Pathogen Pflanzen-Pathogene Protozoa Viren Viruses electron microscopy fungi plant pathogens virus

Editors and affiliations

  • Kurt Mendgen
    • 1
  • Dietrich-Eckhardt Lesemann
    • 2
  1. 1.Fakultät für Biologie, Lehrstuhl PhytopathologieUniversität KonstanzKonstanzGermany
  2. 2.Biologische Bundesanstalt für Land- und ForstwirtschaftInstitut für Viruskrankheiten der PlanzenBraunschweigGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-75818-8
  • Copyright Information Springer-Verlag Berlin Heidelberg 1991
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-75820-1
  • Online ISBN 978-3-642-75818-8
  • Buy this book on publisher's site
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