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Ultraviolet Spectroscopy of Proteins

  • Book
  • © 1986

Overview

Authors:
  1. Alexander P. Demchenko

    1. Palladin Institute of Biochemistry, Academy of Sciences of the Ukraine, Kiev, USSR

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Table of contents (15 chapters)

  1. Front Matter

    Pages I-XI
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  2. Introduction

    • Alexander P. Demchenko
    Pages 1-4

  3. Spectroscopic Properties of Protein Chromophores

    • Alexander P. Demchenko
    Pages 5-26

  4. Display of Intramolecular and Intermolecular Interactions in Electronic Spectra of Amino Acids and Proteins

    • Alexander P. Demchenko
    Pages 27-63

  5. Difference Spectra of Proteins

    • Alexander P. Demchenko
    Pages 64-90

  6. Thermal Perturbation Difference Spectroscopy and Temperature-Dependent Conformational Transitions of Proteins

    • Alexander P. Demchenko
    Pages 91-120

  7. Derivative Spectroscopy of Aromatic Amino Acids and Proteins

    • Alexander P. Demchenko
    Pages 121-136

  8. Spectrophotometric Titration of Proteins

    • Alexander P. Demchenko
    Pages 137-144

  9. Fluorescence Molecular Relaxation Spectroscopy

    • Alexander P. Demchenko
    Pages 145-172

  10. Fluorescence Quenching

    • Alexander P. Demchenko
    Pages 173-182

  11. Nonradiative Transfer of Electronic Excitation Energy

    • Alexander P. Demchenko
    Pages 183-197

  12. Fluorescence Polarization and Rotational Mobility

    • Alexander P. Demchenko
    Pages 198-207

  13. Intrinsic Phosphorescence of Proteins

    • Alexander P. Demchenko
    Pages 208-221

  14. Employment of Ultraviolet Spectroscopy in Analytical Chemistry of Proteins

    • Alexander P. Demchenko
    Pages 222-240

  15. Experimental Technique in Protein Spectroscopy

    • Alexander P. Demchenko
    Pages 241-262

  16. General Conclusions and Prospects

    • Alexander P. Demchenko
    Pages 263-278

  17. Back Matter

    Pages 279-312
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Keywords

About this book

The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.

Authors and Affiliations

  • Palladin Institute of Biochemistry, Academy of Sciences of the Ukraine, Kiev, USSR

    Alexander P. Demchenko

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