Gigaseal Formation in Patch Clamping

With Applications of Nanotechnology

  • Majid Malboubi
  • Kyle Jiang

Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Majid Malboubi, Kyle Jiang
    Pages 1-2
  3. Majid Malboubi, Kyle Jiang
    Pages 3-16
  4. Majid Malboubi, Kyle Jiang
    Pages 17-28
  5. Majid Malboubi, Kyle Jiang
    Pages 29-47
  6. Majid Malboubi, Kyle Jiang
    Pages 49-59
  7. Majid Malboubi, Kyle Jiang
    Pages 61-73

About this book

Introduction

This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.

Keywords

Gigaseal Formation using micropipettes High Resistance Seals High throughput screening Hydrophilicity Nanotechnology Patch Clamp Patch clamping Surface Roughness Tip Size

Authors and affiliations

  • Majid Malboubi
    • 1
  • Kyle Jiang
    • 2
  1. 1.London Centre for NanotechnologyLondonUnited Kingdom
  2. 2.School of Mechanical EngineeringThe University of BirminghamBirminghamUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-39128-6
  • Copyright Information The Author(s) 2014
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Engineering
  • Print ISBN 978-3-642-39127-9
  • Online ISBN 978-3-642-39128-6
  • Series Print ISSN 2191-530X
  • Series Online ISSN 2191-5318
  • About this book
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Pharma
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Engineering