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  • © 2013

Analyzing Microbes

Manual of Molecular Biology Techniques

  • Provides step-by-step instructions for immediate use in the laboratory
  • With flow charts of all experiments
  • Includes suppliers list and recipes of solutions and media
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Microbial DNA Extraction, Purification, and Quantitation

    • Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 1-16
  3. Restriction Enzymes and Their Role in Microbiology

    • Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 31-36
  4. Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis: Methods and Principles

    • Sukumar Mesapogu, Chandra Mouleswararao Jillepalli, Dilip K. Arora
    Pages 73-91
  5. Molecular Identification of Microbes: I. Macrophomina phaseolina

    • Bandamaravuri Kishore Babu, T. Kiran Babu, Rajan Sharma
    Pages 93-97
  6. Molecular Identification of Microbes: III. Pseudomonas

    • Bhim Pratap Singh, Ratul Saikia
    Pages 105-112
  7. Molecular Identification of Microbes: IV. Vibrio

    • Hirak Ranjan Dash, Neelam Mangwani, Surajit Das
    Pages 113-122
  8. Molecular Identification of Microbes: V. methylotrophs

    • Kamlesh K. Meena, Manish Kumar, D. P. Singh
    Pages 123-134
  9. Preservation and Maintenance of Microbial Cultures

    • Sudheer Kumar, Prem L. Kashyap, Ruchi Singh, Alok K. Srivastava
    Pages 135-152
  10. ELISA-Based Identification and Detection of Microbes

    • Jyoti Verma, Sangeeta Saxena, Sunil G. Babu
    Pages 169-186
  11. Analysis of Microbial Diversity and Construction of Metagenomic Library

    • Thangamani Rajesh, Jeyaprakash Rajendhran, Paramasamy Gunasekaran
    Pages 187-208
  12. Bioinformatics Tools for Interpretation of Data Used in Molecular Identification

    • Suchi Smita, Krishna P. Singh, Bashir A. Akhoon, Shishir K. Gupta, Shailendra K. Gupta
    Pages 209-243
  13. Molecular Phylogenetics of Microbes

    • Surajit Das, Hirak Ranjan Dash
    Pages 245-260
  14. Microarray Analysis of Different Functional Genes of Microorganisms

    • Hirak Ranjan Dash, Surajit Das
    Pages 281-290
  15. DNA Cloning and Sequencing

    • Bandamaravuri Kishore Babu, Anu Sharma, Hari Kishan Sudini
    Pages 291-302

About this book

This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.

Reviews

From the reviews:

“The Analyzing Microbes­—Manual of Molecular Biology Techniques is indeed a practical guide to the application of various molecular biology techniques in general microbiological research, with special emphasis in/on biotechnology and environmental microbiology. … All chapters are written in a concise step-by-step algorithm and are easy to follow during reading or bench working. … In my opinion, the aim of this book … to produce a self-contained laboratory manual which will be useful to both experienced practitioners and beginners in the field, is fully accomplished.” (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)

Editors and Affiliations

  • National Bureau of Agriculturally Import, Maunath Bhanjan, India

    Dilip Kumar Arora, Mesapogu Sukumar

  • , Dep. Life Sciences, National Institute of Technology, Rourkela, India

    Surajit Das

Bibliographic Information

  • Book Title: Analyzing Microbes

  • Book Subtitle: Manual of Molecular Biology Techniques

  • Editors: Dilip Kumar Arora, Surajit Das, Mesapogu Sukumar

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-642-34410-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2013

  • Hardcover ISBN: 978-3-642-34409-1Published: 03 February 2013

  • Softcover ISBN: 978-3-662-50967-8Published: 24 September 2016

  • eBook ISBN: 978-3-642-34410-7Published: 03 February 2013

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XI, 352

  • Number of Illustrations: 18 b/w illustrations, 62 illustrations in colour

  • Topics: Microbiology, Medical Microbiology, Biochemistry, general

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access