Antibody Engineering

  • Roland Kontermann
  • Stefan Dübel

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Cloning of the Antigen-binding Site from Hybridoma

    1. Front Matter
      Pages 1-1
    2. Nina Strebe, Frank Breitling, Dieter Moosmayer, Bodo Brocks, Stefan Dübel
      Pages 3-14
    3. Andrew Bradbury
      Pages 15-20
    4. Jonas V. Schaefer, Annemarie Honegger, Andreas Plückthun
      Pages 21-44
  3. Generation of Antibody Repertoires

    1. Front Matter
      Pages 43-43
    2. Christian Kellner, Sahar Mohseni Nodehi, Matthias Peipp
      Pages 47-63
    3. Michael Hust, Stefan Dübel
      Pages 65-84
    4. Pierre Martineau
      Pages 85-97
    5. Thibaut Pelat, Michael Hust, Philippe Thullier
      Pages 99-114
    6. Rüdiger Ridder, Hermann Gram
      Pages 115-123
  4. Selection of Antibody Fragments from Combinatorial Libraries

    1. Front Matter
      Pages 128-128
    2. Roland E. Kontermann
      Pages 127-137
    3. Michael Hust, Michael Mersmann
      Pages 139-149
    4. Patrick Chames, Daniel Baty
      Pages 151-164
    5. Steffen U. Eisenhardt, Karlheinz Peter
      Pages 165-182
    6. Frank Breitling, Olaf Broders, Saskia Helmsing, Michael Hust, Stefan Dübel
      Pages 197-206
    7. Kelly Davis Orcutt, K. Dane Wittrup
      Pages 207-233
    8. Xiangang Zou, Guocheng Lan, Michael J. Osborn, Marianne Brüggemann
      Pages 235-254

About this book


Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity.

Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.


Antigen Cancer therapy Chloroplast Expression Hybridoma Immunoglobulins Immunology Polymerasekettenreaktion Protein engineering chromatography cloning diagnosis mutagenesis protein transgen

Editors and affiliations

  • Roland Kontermann
    • 1
  • Stefan Dübel
    • 2
  1. 1.(Biomedical Engineering) Institut für Zellbiologie und ImmunologieUniversität StuttgartStuttgartGermany
  2. 2.Technische Universität BraunschweigInstitut für Biochemie und BiotechnologieGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2010
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-642-01143-6
  • Online ISBN 978-3-642-01144-3
  • About this book

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