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Cellular Imaging

Electron Tomography and Related Techniques

Editors:

Part of the book series: Biological and Medical Physics, Biomedical Engineering (BIOMEDICAL)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xix
  2. Electron Tomography: A Primer

    • Andrew Leis
    Pages 1-32
  3. STEM Tomography in Biology

    • Sharon Grayer Wolf, Eyal Shimoni, Michael Elbaum, Lothar Houben
    Pages 33-60
  4. Electron Cryo-Tomography

    • Josie L. Ferreira, Teige R. S. Matthews-Palmer, Morgan Beeby
    Pages 61-94
  5. Large-Scale Electron Tomography of Cells Using SerialEM and IMOD

    • Eileen O’Toole, Peter van der Heide, J. Richard McIntosh, David Mastronarde
    Pages 95-116
  6. Alignment of Tilt Series

    • A. Verguet, C. Messaoudi, C. O. S. Sorzano, S. Marco
    Pages 183-207
  7. Tomographic Reconstruction from Electron Micrographs

    • J. Bernard Heymann
    Pages 209-236
  8. Structural Biology in Situ Using Cryo-Electron Subtomogram Analysis

    • Stefan Pfeffer, Friedrich Förster
    Pages 237-259
  9. Resolution in Electron Tomography

    • Mikhail Kudryashev
    Pages 261-282
  10. Signal Optimization in Electron Tomography

    • Mauro Maiorca, Peter B. Rosenthal
    Pages 283-300
  11. Back Matter

    Pages 319-323

About this book

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). 


The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation


Editors and Affiliations

  • Bio21 Institute Advanced Microscopy Facility, University of Melbourne, Melbourne, Australia

    Eric Hanssen

Bibliographic Information

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access