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  • © 2016

The Next Generation in Membrane Protein Structure Determination

Editors:

  • Gives novel approaches to structure determination of membrane proteins

  • Provides good coverage on how membrane proteins interact with their surrounding environment

  • Is a useful guide not only for students, but also for experienced scientists who need to deal with very small and problematic protein crystals

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 922)

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eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-35072-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)

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Table of contents (14 chapters)

  1. Front Matter

    Pages i-x
  2. Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters

    • Louise E. Bird, Joanne E. Nettleship, Valtteri J√§rvinen, Heather Rada, Anil Verma, Raymond J. Owens
    Pages 1-11
  3. Detergents in Membrane Protein Purification and Crystallisation

    • Anandhi Anandan, Alice Vrielink
    Pages 13-28
  4. NMR of Membrane Proteins: Beyond Crystals

    • Sundaresan Rajesh, Michael Overduin, Boyan B. Bonev
    Pages 29-42
  5. Membrane Protein Crystallisation: Current Trends and Future Perspectives

    • Joanne L. Parker, Simon Newstead
    Pages 61-72Open Access
  6. Crystal Dehydration in Membrane Protein Crystallography

    • Juan Sanchez-Weatherby, Isabel Moraes
    Pages 73-89Open Access
  7. Exploiting Microbeams for Membrane Protein Structure Determination

    • Anna J. Warren, Danny Axford, Neil G. Paterson, Robin L. Owen
    Pages 105-117Open Access
  8. Applications of the BLEND Software to Crystallographic Data from Membrane Proteins

    • Pierre Aller, Tian Geng, Gwyndaf Evans, James Foadi
    Pages 119-135Open Access
  9. Serial Millisecond Crystallography of Membrane Proteins

    • Kathrin Jaeger, Florian Dworkowski, Przemyslaw Nogly, Christopher Milne, Meitian Wang, Joerg Standfuss
    Pages 137-149
  10. Serial Femtosecond Crystallography of Membrane Proteins

    • Lan Zhu, Uwe Weierstall, Vadim Cherezov, Wei Liu
    Pages 151-160
  11. Beyond Membrane Protein Structure: Drug Discovery, Dynamics and Difficulties

    • Philip C. Biggin, Matteo Aldeghi, Michael J. Bodkin, Alexander Heifetz
    Pages 161-181
  12. Erratum to: Applications of the BLEND Software to Crystallographic Data from Membrane Proteins

    • Pierre Aller, Tian Geng, Gwyndaf Evans, James Foadi
    Pages E3-E3Open Access
  13. Back Matter

    Pages 183-184

About this book

This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects.  Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins.

This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes:

  • Expression screening using fluorescent proteins
  • The use of detergents in membrane protein research
  • The use of NMR
  • Synchrotron developments in membrane protein structural biology
  • Visualisation and X-ray data collection of microcrystals
  • X-ray diffraction data analysis from multiple crystals
  • Serial millisecond crystallography
  • Serial femtosecond crystallography
  • Membrane protein structures in drug discovery

The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work.

Keywords

  • Crystallization
  • XFEL
  • Molecular dynamics
  • Human genome
  • Electron Lasers
  • Protein Structure

Editors and Affiliations

  • Membrane Protein Laboratory, Diamond Light Source/Imperial College London, Didcot, UK

    Isabel Moraes

Bibliographic Information

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-35072-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.99
Price excludes VAT (USA)
Hardcover Book USD 179.99
Price excludes VAT (USA)