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Plant Microtechniques and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Edward Chee Tak Yeung

    1. Department of Biological Sciences, University of Calgary, CALGARY, Canada

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  2. Claudio Stasolla

    1. Department of Plant Sciences, University of Manitoba, Winnipeg, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Michael John Sumner

    1. Department of Biological Sciences, University of Manitoba, Winnipeg, Canada

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  4. Bing Quan Huang

    1. Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

  • The first comprehensive work of microtechniques of botanical specimens
  • Detailed analysis of various microtechnique protocols of plant specimen studies
  • Clear and easy to understand procedures for various specimen analyses Written by leading scholars from around the world
  • Includes supplementary material: sn.pub/extras

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Table of contents (30 chapters)

  1. Microtechniques Related to Cell and Molecular Biology Studies

    1. Detection of S-Phase of Cell Division Cycle in Plant Cells and Tissues by Using 5-Ethynyl-2′-Deoxyuridine (EdU)

      • Soujanya Kuntam, Ferhan Ayaydin
      Pages 311-322

    2. Staining Methods for Programmed Cell Death

      • Owen S. D. Wally, Claudio Stasolla
      Pages 323-336

    3. Laser Microdissection of Plant Tissues

      • Jenna L. Millar, Michael G. Becker, Mark F. Belmonte
      Pages 337-350

    4. RNA In situ Hybridization

      • Shalini Mukherjee, Claudio Stasolla, Belay T. Ayele
      Pages 351-370

  2. Preparative Methods for the Study of Wood Anatomy

    1. Front Matter

      Pages 371-371
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    2. Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy

      • Jacques C. Tardif, France Conciatori
      Pages 373-415

    3. Collecting and Processing Wood Microcores for Monitoring Xylogenesis

      • Annie Deslauriers, Sergio Rossi, Eryuan Liang
      Pages 417-429

    4. Three-Dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy

      • Satoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho et al.
      Pages 431-465

  3. Botanical Techniques and Protocols for Archeology and Herbarium Collection

    1. Front Matter

      Pages 467-467
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    2. Collection of Plant Remains from Archaeological Contexts

      • Alessandra Celant, Donatella Magri, Francesca Romana Stasolla
      Pages 469-485

    3. Archaeological Wood Preparation

      • Alessandra Celant, Gemma Coccolini
      Pages 487-493

    4. Archaeopalynological Preparation Techniques

      • Donatella Magri, Federico Di Rita
      Pages 495-506

    5. Phytoliths: Preparation and Archaeological Extraction

      • Brian Kooyman
      Pages 507-524

    6. Starch Granules: Preparation and Archaeological Extraction

      • Brian Kooyman
      Pages 525-540

    7. Plant Collection, Identification, and Herbarium Procedures

      • Bonnie Smith, C.C. Chinnappa
      Pages 541-572

  4. Back Matter

    Pages 573-576
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Keywords

About this book

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Editors and Affiliations

  • Department of Biological Sciences, University of Calgary, CALGARY, Canada

    Edward Chee Tak Yeung

  • Department of Plant Sciences, University of Manitoba, Winnipeg, Canada

    Claudio Stasolla

  • Department of Biological Sciences, University of Manitoba, Winnipeg, Canada

    Michael John Sumner

  • Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, USA

    Bing Quan Huang

About the editors

Edward C. Yeung
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

Claudio Stasolla
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, Canada

Michael J. Sumner
Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

Bing Q. Huang
Mayo Clinic, Rochester, Minnesota, USA

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