Genetic Transformation Systems in Fungi, Volume 1

  • Marco A. van den Berg
  • Karunakaran Maruthachalam

Part of the Fungal Biology book series (FUNGBIO)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

  3. Transformation Methods: Protoplast Transformation

    1. Front Matter
      Pages 19-19
    2. Aroa Rodriguez-Iglesias, Monika Schmoll
      Pages 21-40
    3. Mónica G. Malmierca, Rosa E. Cardoza, Santiago Gutiérrez
      Pages 41-48
    4. Victoriano Garre, José Luis Barredo, Enrique A. Iturriaga
      Pages 49-59
    5. Shigeyuki Kawai, Kousaku Murata
      Pages 61-63
  4. Transformation Methods: Electroporation

    1. Front Matter
      Pages 65-65
    2. Sophie Bachellier-Bassi, Christophe d’Enfert
      Pages 81-85
    3. Knut Madden, Ilya Tolstorukov, James Cregg
      Pages 87-91
  5. Transformation Methods: Particle Bombardment

    1. Front Matter
      Pages 99-99
    2. Arianna Montanari, Monique Bolotin-Fukuhara, Mario Fazzi D’Orsi, Cristina De Luca, Michele M. Bianchi, Silvia Francisci
      Pages 101-117
    3. Jingwen Zhou, Liming Liu, Guocheng Du, Jian Chen
      Pages 119-127
    4. V. S. Junior Te’o, K. M. Helena Nevalainen
      Pages 129-133
    5. Eiji Sakuradani, Hiroshi Kikukawa, Seiki Takeno, Akinori Ando, Sakayu Shimizu, Jun Ogawa
      Pages 135-140
  6. Transformation Methods: Agrobacterium-Mediated Transformation

    1. Front Matter
      Pages 141-141
    2. Rasmus John Normand Frandsen
      Pages 143-162
    3. Manish Pareek, Mahak Sachdev, Meenakshi Tetorya, Manchikatla V. Rajam
      Pages 169-174
  7. Transformation Methods: Li-acetate Transformation

  8. Transformation Methods: New Developments

    1. Front Matter
      Pages 199-199
    2. Yevhen Filyak, Nataliya Finiuk, Nataliya Mitina, Alexander Zaichenko, Rostyslav Stoika
      Pages 201-207
    3. Miguel A. Gómez-Lim, Denis Magaña Ortíz, Francisco Fernández, Achim M. Loske
      Pages 209-219
  9. Exogenous DNA: Uptake of DNA

  10. Exogenous DNA: Integration of DNA

    1. Front Matter
      Pages 253-253
    2. Keiichiro Suzuki, Hirokazu Inoue
      Pages 255-262
    3. Aurin M. Vos, Luis G. Lugones, Han A. B. Wösten
      Pages 273-287
    4. Ting Li, David A. Wright, Martin H. Spalding, Bing Yang
      Pages 289-307
  11. Back Matter
    Pages 309-318

About this book


Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ​

Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.


fungi protocols reviews tools transformation

Editors and affiliations

  • Marco A. van den Berg
    • 1
  • Karunakaran Maruthachalam
    • 2
  1. 1.Applied Biochemistry DepartmentDSM Biotechnology CenterDelftThe Netherlands
  2. 2.Global Marker Technology Lab (DuPont-Pioneer)E.I.DuPont India Pvt Ltd, DuPont Knowledge CenterHyderabad, TelanganaIndia

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