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Plant-Pathogen Interactions

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Paul Birch

    1. Dundee Effector Consortium, Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

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  2. John T. Jones

    1. Cell and Molecular Sciences,The James Hutton Institute, Dundee, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  3. Jorunn I.B. Bos

    1. Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1127)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Bioinformatics

    1. Front Matter

      Pages 1-1
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    2. Galaxy as a Platform for Identifying Candidate Pathogen Effectors

      • Peter J. A. Cock, Leighton Pritchard
      Pages 3-15

    3. Bioinformatic Analysis of Expression Data to Identify Effector Candidates

      • Adam J. Reid, John T. Jones
      Pages 17-27

    4. Two-Dimensional Data Binning for the Analysis of Genome Architecture in Filamentous Plant Pathogens and Other Eukaryotes

      • Diane G. O. Saunders, Joe Win, Sophien Kamoun, Sylvain Raffaele
      Pages 29-51

    5. On the Statistics of Identifying Candidate Pathogen Effectors

      • Leighton Pritchard, David Broadhurst
      Pages 53-64

  3. Cell Biology

    1. Front Matter

      Pages 65-65
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    2. High-Throughput Imaging of Plant Immune Responses

      • Martina Beck, Ji Zhou, Christine Faulkner, Silke Robatzek
      Pages 67-80

    3. In Vivo Protein–Protein Interaction Studies with BiFC: Conditions, Cautions, and Caveats

      • Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, Paul Birch
      Pages 81-90

    4. Particle Bombardment-Mediated Transient Expression to Identify Localization Signals in Plant Disease Resistance Proteins and Target Sites for the Proteolytic Activity of Pathogen Effectors

      • Daigo Takemoto, David A. Jones
      Pages 91-101

    5. Purification of Fungal Haustoria from Infected Plant Tissue by Flow Cytometry

      • Diana P. Garnica, John P. Rathjen
      Pages 103-110

  4. From Assays of Pathogen Virulence to Effector Function

    1. Front Matter

      Pages 111-111
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    2. Functional Characterization of Nematode Effectors in Plants

      • Axel A. Elling, John T. Jones
      Pages 113-124

    3. Silencing of Aphid Genes by Feeding on Stable Transgenic Arabidopsis thaliana

      • Alexander D. Coleman, Marco Pitino, Saskia A. Hogenhout
      Pages 125-136

    4. Leaf-Disc Assay Based on Transient Over-Expression in Nicotiana benthamiana to Allow Functional Screening of Candidate Effectors from Aphids

      • Patricia A. Rodriguez, Saskia A. Hogenhout, Jorunn I. B. Bos
      Pages 137-143

    5. A Growth Quantification Assay for Hyaloperonospora arabidopsidis Isolates in Arabidopsis thaliana

      • Daniel F. A. Tomé, Jens Steinbrenner, Jim L. Beynon
      Pages 145-158

    6. Simple Quantification of In Planta Fungal Biomass

      • Michael Ayliffe, Sambasivam K. Periyannan, Angela Feechan, Ian Dry, Ulrike Schumann, Evans Lagudah et al.
      Pages 159-172

    7. Virus-Induced Gene Silencing and Agrobacterium tumefaciens-Mediated Transient Expression in Nicotiana tabacum

      • Zhao Zhang, Bart P. H. J. Thomma
      Pages 173-181

    8. DIGE-ABPP by Click Chemistry: Pairwise Comparison of Serine Hydrolase Activities from the Apoplast of Infected Plants

      • Tram Ngoc Hong, Renier A. L. van der Hoorn
      Pages 183-194

    9. A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector: Host Protein Complexes

      • Jens Steinbrenner, Matthew Eldridge, Daniel F. A. Tomé, Jim L. Beynon
      Pages 195-211
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Keywords

About this book

Plant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify and characterize effectors and to study their impacts on host immunity and their roles in pathogen biology. Additional chapters focus on protocols to identify avirulence and resistance genes, investigate the roles of effector targets and other defence-associated proteins in plant immunity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant-Pathogen Interactions: Methods and Protocols, Second Edition seeks to aid scientists in the further study of plant immunity.

Editors and Affiliations

  • Dundee Effector Consortium, Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

    Paul Birch

  • Cell and Molecular Sciences,The James Hutton Institute, Dundee, United Kingdom

    John T. Jones

  • Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

    Jorunn I.B. Bos

Bibliographic Information

  • Book Title: Plant-Pathogen Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Birch, John T. Jones, Jorunn I.B. Bos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-986-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-985-7Published: 19 March 2014

  • Softcover ISBN: 978-1-4939-6323-2Published: 24 September 2016

  • eBook ISBN: 978-1-62703-986-4Published: 18 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 306

  • Number of Illustrations: 14 b/w illustrations, 43 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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