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Polyadenylation

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Joanna Rorbach

    1. MRC Mitochondrial Biology Unit, Mitochondrial Genetics Group, Cambridge, United Kingdom

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  2. Agnieszka J. Bobrowicz

    1. Mitochondrial Research Group, Newcastle University The Medical School, Newcastle upon Tyne, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1125)

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Table of contents (30 protocols)

  1. Bacteria and Organelles

    1. Polyadenylation in Bacteria and Organelles

      • Joanna Rorbach, Agnieszka Bobrowicz, Sarah Pearce, Michal Minczuk
      Pages 211-227

    2. In Vivo Analysis of Polyadenylation in Prokaryotes

      • Bijoy K. Mohanty, Sidney R. Kushner
      Pages 229-249

    3. Polyadenylation of RNA in E. coli: RNA Polymerase-Associated (rA)n-Synthetic Activities

      • Grant A. Encalade, Maxim V. Sukhodolets
      Pages 251-262

    4. Analysis of the Human Mitochondrial Transcriptome Using Directional Deep Sequencing and Parallel Analysis of RNA Ends

      • Oliver Rackham, Aleksandra Filipovska
      Pages 263-275

    5. Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine

      • Lukasz S. Borowski, Roman J. Szczesny
      Pages 277-286

  2. Deadenylation and Decay

    1. Front Matter

      Pages 287-287
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    2. Deadenylation and Its Regulation in Eukaryotic Cells

      • Xiaokan Zhang, Frida E. Kleiman, Emral Devany
      Pages 289-296

    3. Assaying mRNA Deadenylation In Vitro

      • Mandy Jeske, Claudia Temme, Elmar Wahle
      Pages 297-311

    4. Assaying mRNA Deadenylation In Vivo

      • Claudia Temme, Elmar Wahle
      Pages 313-324

    5. In Vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes

      • Joel Hrit, Nathan Raynard, Jamie Van Etten, Kamya Sankar, Adam Petterson, Aaron C. Goldstrohm
      Pages 325-339

    6. MicroRNA-Mediated Deadenylation in a Mammalian Cell-Free System

      • Motoaki Wakiyama, Shigeyuki Yokoyama
      Pages 341-351

    7. Quantitative Analysis of Deadenylation-Independent mRNA Decay by a Modified MBRACE Assay

      • Julie A. Dougherty, Roshan Mascarenhas, Daniel R. Schoenberg
      Pages 353-371

  3. Erratum To: Cytoplasmic Polyadenylation Assays

    • Olga Coll, Ana Villalba, Fátima Gebauer
    Pages E1-E1

  4. Erratum: Retracted: Poly(A) Polymerase-Based Poly(A) Length Assay

    • Deepak P. Patil, Baskar Bakthavachalu, Daniel R. Schoenberg
    Pages E3-E3

  5. Back Matter

    Pages 373-377
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Keywords

About this book

In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • MRC Mitochondrial Biology Unit, Mitochondrial Genetics Group, Cambridge, United Kingdom

    Joanna Rorbach

  • Mitochondrial Research Group, Newcastle University The Medical School, Newcastle upon Tyne, United Kingdom

    Agnieszka J. Bobrowicz

Bibliographic Information

  • Book Title: Polyadenylation

  • Book Subtitle: Methods and Protocols

  • Editors: Joanna Rorbach, Agnieszka J. Bobrowicz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-971-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-62703-970-3Published: 04 March 2014

  • Softcover ISBN: 978-1-4939-6055-2Published: 03 September 2016

  • eBook ISBN: 978-1-62703-971-0Published: 28 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 377

  • Number of Illustrations: 41 b/w illustrations, 22 illustrations in colour

  • Topics: Human Genetics, Gene Function

  • Industry Sectors: Biotechnology, Pharma

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