Electron Microscopy

Methods and Protocols

  • John Kuo

Part of the Methods in Molecular Biology book series (MIMB, volume 1117)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Gregory M. Hendricks
    Pages 73-93
  3. Douglas E. Chandler, William P. Sharp
    Pages 95-132
  4. Andres Kaech, Urs Ziegler
    Pages 151-171
  5. Markus Grabenbauer, Hong-Mei Han, Jan Huebinger
    Pages 173-191
  6. Petr Chlanda, Martin Sachse
    Pages 193-214
  7. Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison
    Pages 259-271
  8. Christian Hacker, John M. Lucocq
    Pages 315-323
  9. Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, Henning Stahlberg
    Pages 325-341
  10. Dušan Cmarko, Anna Ligasová, Karel Koberna
    Pages 343-366
  11. Yuri L. Lyubchenko, Alexander A. Gall, Luda S. Shlyakhtenko
    Pages 367-384

About this book


This third edition of Electron Microscopy: Methods and Protocols  expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods.  With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation,  negative staining and immunogold labelling techniques,  DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy,  TEM crystallography and cryoTEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM , Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM, and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and NanoSIMS. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical,  Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.


(EM) data Cryo-specimen DNA and RNA tracking Focussed Ion Beam (FIB) Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) tomography bio-molecular electron microscopy bio-molecular research correlative microscopy cryoTEM 3D tomography electron microscopes

Editors and affiliations

  • John Kuo
    • 1
  1. 1.University of Western AustraliaCrawleyAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-62703-776-1
  • Copyright Information Springer Science+Business Media, New York 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-775-4
  • Online ISBN 978-1-62703-776-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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