Editors:
- Summarizes methods for preparation and purification of modified peptides
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1081)
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Table of contents (13 protocols)
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Front Matter
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Back Matter
About this book
In Peptide Modifications to Increase Metabolic Stability and Activity, expert researchers in the field provide summarized methods for preparation, purification of modified peptides, and assessment of their biochemical activities. These methods and protocols include preparation of conformationally constrained peptides, modification of peptide bonds, introduction of nonproteinogenic amino acids, and alteration of peptides’ physical and biological properties by modification of the amino acid side chains and/or terminal residues. With additional chapter that describes new experimental approach for the detection of exogenous peptides within living cells using peptides labeled with heavy isotopes and confocal Raman microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Peptide Modifications to Increase Metabolic Stability and Activity seeks to provide scientists with alternative approaches to peptide modification that many researchers may find applicable to their specific research requirements.
Editors and Affiliations
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Torrey Pines Institute for Molecular Stu, Port St. Lucie, USA
Predrag Cudic
Bibliographic Information
Book Title: Peptide Modifications to Increase Metabolic Stability and Activity
Editors: Predrag Cudic
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-652-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media Newyork 2013
Hardcover ISBN: 978-1-62703-651-1Published: 07 September 2013
Softcover ISBN: 978-1-4939-6284-6Published: 23 August 2016
eBook ISBN: 978-1-62703-652-8Published: 07 September 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: IX, 241
Number of Illustrations: 76 b/w illustrations, 16 illustrations in colour
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma