Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1072)
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Table of contents (53 protocols)
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Front Matter
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Introductory
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Front Matter
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Experimental
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Front Matter
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About this book
Plant Proteomics: Methods and Protocols, Second Edition presents recent advances made in the field of proteomics and their application to plant biology and translational research. In recent years, improvements in techniques and protocols for high-throughput proteomics have been made at all workflow stages, from wet (sampling, tissue and cell fractionation, protein extraction, depletion, purification, separation, MS analysis, quantification) to dry lab (experimental design, algorithms for protein identification, bioinformatics tools for data analysis, databases, and repositories). Divided into nine convenient sections, chapters cover topics such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, as well as the study and analysis of PTMs, protein interactions, and specific families of proteins, and finally proteomics in translational research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Plant Proteomics: Methods and Protocols, Second Edition seeks to serve both professionals and novices looking to exploit the full potential of proteomics in plant biology research.
Editors and Affiliations
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Dept of Biochem & Molecular Bio, University of Cordoba, Cordoba, Spain
Jesus V. Jorrin-Novo
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University of Tsukuba National Institute of Crop Science, Tsukuba, Japan
Setsuko Komatsu
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Molecular Systems Biology, University of Vienna, Vienna, Austria
Wolfram Weckwerth
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Dept. Molecular Systems Biology, University of Vienna, Vienna, Austria
Stefanie Wienkoop
Bibliographic Information
Book Title: Plant Proteomics
Book Subtitle: Methods and Protocols
Editors: Jesus V. Jorrin-Novo, Setsuko Komatsu, Wolfram Weckwerth, Stefanie Wienkoop
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-631-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-630-6Published: 18 October 2013
Softcover ISBN: 978-1-4939-6029-3Published: 17 September 2016
eBook ISBN: 978-1-62703-631-3Published: 17 October 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XXI, 786
Number of Illustrations: 54 b/w illustrations, 75 illustrations in colour
Topics: Plant Sciences, Proteomics
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma