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Jasmonate Signaling

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Alain Goossens

    1. Department of Plant Systems Biology, VIB, Gent, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Laurens Pauwels

    1. Department of Plant Systems Biology, VIB, Gent, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Combines cutting-edge omics protocols with more common reductionist approaches to the study of jasmonate signaling pathways
  • Features clear, step-by-step methodologies provided by experts in the field
  • Includes key tips and vital implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1011)

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Table of contents (26 protocols)

  1. Molecules

    1. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA

      • Johan Memelink
      Pages 209-225

    2. Transient Expression Assays in Tobacco Protoplasts

      • Robin Vanden Bossche, Brecht Demedts, Rudy Vanderhaeghen, Alain Goossens
      Pages 227-239

    3. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters

      • Nobukazu Shitan, Akifumi Sugiyama, Kazufumi Yazaki
      Pages 241-250

    4. Expression Analysis of Jasmonate-Responsive Lectins in Plants

      • Nausicaä Lannoo, Els J. M. Van Damme
      Pages 251-263

  2. Omics

    1. Front Matter

      Pages 265-265
      Download chapter PDF

    2. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics

      • Hye Kyong Kim, Young Hae Choi, Robert Verpoorte
      Pages 267-275

    3. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

      • Jacob Pollier, Alain Goossens
      Pages 277-286

    4. cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures

      • Janine Colling, Jacob Pollier, Nokwanda P. Makunga, Alain Goossens
      Pages 287-303

    5. Analysis of RNA-Seq Data with TopHat and Cufflinks for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures

      • Jacob Pollier, Stephane Rombauts, Alain Goossens
      Pages 305-315

    6. Transcriptome Coexpression Analysis Using ATTED-II for Integrated Transcriptomic/Metabolomic Analysis

      • Keiko Yonekura-Sakakibara, Kazuki Saito
      Pages 317-326

    7. A Guide to CORNET for the Construction of Coexpression and Protein–Protein Interaction Networks

      • Stefanie De Bodt, Dirk Inzé
      Pages 327-343

  3. Back Matter

    Pages 345-347
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Keywords

About this book

It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment.  In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants.  Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Editors and Affiliations

  • Department of Plant Systems Biology, VIB, Gent, Belgium

    Alain Goossens, Laurens Pauwels

Bibliographic Information

  • Book Title: Jasmonate Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Alain Goossens, Laurens Pauwels

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-414-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-413-5Published: 25 April 2013

  • Softcover ISBN: 978-1-4939-6013-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-414-2Published: 08 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 347

  • Number of Illustrations: 23 b/w illustrations, 33 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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