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  • Book
  • © 2013

Plant Lipid Signaling Protocols

  • Presents timely and important tools needed to elucidate particular roles of lipid (-derived) signals in plants
  • Includes easy-to-use, reproducible protocols
  • Contains expert tips on troubleshooting and avoiding known pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1009)

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eBook USD 59.99
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Softcover Book USD 79.99
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Hardcover Book USD 109.99
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Table of contents (27 protocols)

  1. Enzyme Activities

    1. Assaying Nonspecific Phospholipase C Activity

      • PÅ™emysl Pejchar, Günther F. E. Scherer, Jan Martinec
      Pages 193-203
    2. Measuring PLD Activity In Vivo

      • Teun Munnik, Ana M. Laxalt
      Pages 219-231
    3. Assay of Phospholipase A Activity

      • Michael Heinze, Werner Roos
      Pages 241-249
  2. Protein–Lipid Interactions

    1. Front Matter

      Pages 251-251
    2. Lipid-Binding Analysis Using a Fat Blot Assay

      • Teun Munnik, Magdalena Wierzchowiecka
      Pages 253-259
    3. Liposome-Binding Assays to Assess Specificity and Affinity of Phospholipid–Protein Interactions

      • Magdalena M. Julkowska, Johanna M. Rankenberg, Christa Testerink
      Pages 261-271
  3. Back Matter

    Pages 303-305

About this book

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In  Plant Lipid Signaling Protocols,  expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied.  These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.

Editors and Affiliations

  • Swammerdam Inst. Life Science, Sect. Plant Pathology, University of Amsterdam, Amsterdam, The Netherlands

    Teun Munnik

  • Institute for Biochemistry and Biotechno, Department of Cellular Biochemistry, Martin-Luther-Univ Halle-Wittenberg, Halle, Germany

    Ingo Heilmann

Bibliographic Information

  • Book Title: Plant Lipid Signaling Protocols

  • Editors: Teun Munnik, Ingo Heilmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-401-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-400-5Published: 17 May 2013

  • Softcover ISBN: 978-1-4939-5927-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-401-2Published: 17 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 305

  • Number of Illustrations: 22 b/w illustrations, 15 illustrations in colour

  • Topics: Plant Sciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 59.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access