Overview
- Presents detailed protocols on the production and characterization of glycoproteins and glyco-engineered biopharmaceuticals
- Contains a special focus on monoclonal antibodies (mAbs)
- Features expert tips and key implementation advice to guarantee successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB)
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Table of contents (22 protocols)
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PK/PD Assays for Therapeutic Antibodies
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Front Matter
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Back Matter
Keywords
About this book
Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins. In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.
Reviews
From the reviews:
“The book is intended as a reference manual of protocols and best practices for the practicing bench scientist. The authors do a marvelous job of covering a wide variety of tools and techniques and bringing them together in one volume. … This collection of protocols will find an audience with experimentalists in the biopharmaceutical industry as well as various academic labs. … Overall, I would strongly recommend this book … .” (Bibhash Mukhopadhyay, Frontiers in Pharmacology, September, 2013)
“This book … useful as it details in one place the current state of art technologies and techniques for antibodies. I enjoyed reading the introductions to each chapter, which were accessible and gave an historical setting and overall aim for the methodologies that are set out in the chapter. … this book offers a good reference guide for those of us working in this area.” (Sian Estdale, BTS Newsletter – The British Toxicology Society, Issue 43, Winter, 2013)Editors and Affiliations
Bibliographic Information
Book Title: Glycosylation Engineering of Biopharmaceuticals
Book Subtitle: Methods and Protocols
Editors: Alain Beck
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-327-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-326-8Published: 09 March 2013
Softcover ISBN: 978-1-4939-6266-2Published: 23 August 2016
eBook ISBN: 978-1-62703-327-5Published: 12 March 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 355
Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma