Overview
- Covers the entire range of techniques used in electron crystallography, from protein sample preparation to modeling of conformational changes
- Features detailed protocols suitable for labs with minimal equipment and subject familiarity and other techniques for the most state-of-the-art facilities
- Includes key tips and expert implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 955)
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Table of contents (30 protocols)
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Back Matter
Keywords
About this book
The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment. Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods. Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results.
Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.
Reviews
From the reviews:
“This book contains chapters covering all of the key elements of electron crystallography. … This is definitely a valuable book to have in every laboratory that is actively working in the area of electron crystallography because it provides a great deal of information in one convenient place. It would also be useful for anyone who just wants to get a general understanding about this branch of electron microscopy and structural biology, even without intending to use crystals in their own work.” (Robert Glaeser, Microscopy and Microanalysis, Vol. 19 (3), June, 2013)Editors and Affiliations
Bibliographic Information
Book Title: Electron Crystallography of Soluble and Membrane Proteins
Book Subtitle: Methods and Protocols
Editors: Ingeborg Schmidt-Krey, Yifan Cheng
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-176-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-175-2Published: 07 November 2012
Softcover ISBN: 978-1-4939-5876-4Published: 23 August 2016
eBook ISBN: 978-1-62703-176-9Published: 07 November 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 586
Topics: Protein Science, Biological Microscopy
Industry Sectors: Biotechnology, Pharma