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  • © 2013

Basic Cell Culture Protocols

  • Contains numerous new chapters that explore the most current areas of investigation as well as updated chapters from the previous edition
  • Includes clear and concise protocols, ready for the laboratory
  • Features expert tips and key implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 946)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiv
  2. Detection of Mycoplasma Contaminations

    • Cord C. Uphoff, Hans G. Drexler
    Pages 1-13
  3. Eradication of Mycoplasma Contaminations

    • Cord C. Uphoff, Hans G. Drexler
    Pages 15-26
  4. Classical and Molecular Cytogenetic Analysis

    • Roderick A. F. MacLeod, Hans G. Drexler
    Pages 39-60
  5. The Development of T Lymphocytes in Fetal Thymus Organ Culture

    • Takeshi Nitta, Izumi Ohigashi, Yousuke Takahama
    Pages 85-102
  6. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors

    • Génève Awong, Juan Carlos Zúñiga-Pflücker
    Pages 103-113
  7. Isolation and Characterization of Mouse Side Population Cells

    • Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, Margaret A. Goodell
    Pages 151-162
  8. Isolation and Characterization of Cancer Stem Cells In Vitro

    • Craig Gedye, Laurie Ailles
    Pages 181-204
  9. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets

    • Nicolas Pineault, Amélie Robert, Valérie Cortin, Lucie Boyer
    Pages 205-224
  10. Generation and Characterization of Murine Alternatively Activated Macrophages

    • Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, Laura M. Sly
    Pages 225-239
  11. Human Long-Term Culture Initiating Cell Assay

    • Min Liu, Cindy L. Miller, Connie J. Eaves
    Pages 241-256
  12. Long-Term Culture-Initiating Cell Assay for Mouse Cells

    • Stefan Woehrer, Cindy L. Miller, Connie J. Eaves
    Pages 257-266
  13. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells

    • Bert Wognum, Ning Yuan, Becky Lai, Cindy L. Miller
    Pages 267-283
  14. Studying Leukocyte Recruitment Under Flow Conditions

    • Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, Kamala D. Patel
    Pages 285-300
  15. Generation and Establishment of Murine Adherent Cell Lines

    • Rouzanna Istvanffy, Robert A. J. Oostendorp
    Pages 301-314

About this book

At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems.  Written in the highly successful Methods in Molecular Biology™, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Editors and Affiliations

  • , Experimental Therapeutics, BC Cancer Agency, Vancouver, Canada

    Cheryl D. Helgason

  • STEMCELL Technologies, Inc., Vancouver, Canada

    Cindy L. Miller

Bibliographic Information

  • Book Title: Basic Cell Culture Protocols

  • Editors: Cheryl D. Helgason, Cindy L. Miller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-128-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-127-1Published: 20 November 2012

  • Softcover ISBN: 978-1-4939-5652-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-128-8Published: 28 November 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XIV, 550

  • Topics: Cell Biology, Cell Culture

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access