Methods and Protocols

  • Robert Ménard

Part of the Methods in Molecular Biology book series (MIMB, volume 923)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Parasite Culturing

    1. Front Matter
      Pages 1-1
    2. Alexander G. Maier, Melanie Rug
      Pages 3-15
    3. Kathryn Shaw Saliba, Marcelo Jacobs-Lorena
      Pages 17-25
    4. Anil K. Ghosh, Marcelo Jacobs-Lorena
      Pages 27-33
    5. Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, Clemens H. M. Kocken
      Pages 35-49
    6. Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker et al.
      Pages 51-72
  3. Genome Manipulation

    1. Front Matter
      Pages 73-73
    2. Melanie Rug, Alexander G. Maier
      Pages 75-98
    3. Nisha Philip, Rachael Orr, Andrew P. Waters
      Pages 99-125
    4. Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, Oliver Billker
      Pages 127-138
    5. Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, Chris J. Janse
      Pages 139-150
    6. Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer
      Pages 151-159
    7. Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, Stéphanie A. Blandin
      Pages 161-176
  4. “OMIC” Techniques

    1. Front Matter
      Pages 177-177
    2. Sohini Sanyal, Thomas J. Templeton, Cristina K. Moreira
      Pages 179-188
    3. Zbynek Bozdech, Sachel Mok, Archna P. Gupta
      Pages 189-211
    4. Heather J. Painter, Lindsey M. Altenhofen, Björn F. C. Kafsack, Manuel Llinás
      Pages 213-219
    5. Wieteke A. M. Hoeijmakers, Richárd Bártfai, Hendrik G. Stunnenberg
      Pages 221-239
    6. Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, Christian Doerig
      Pages 241-257

About this book


Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of  Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, "omic" approaches, and  techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical,  Malaria: Methods and Protocols, Second Edition offers a comprehensive set of standard techniques for laboratory  researchers.


Plasmodium cellular malariologists culture techniques genome manipulation molecular malariologists parasite culture techniques parasite genome ‘omic’ approaches

Editors and affiliations

  • Robert Ménard
    • 1
  1. 1., Malaria Biology and Genetics UnitInstitut PasteurParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-025-0
  • Online ISBN 978-1-62703-026-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Consumer Packaged Goods