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Malaria

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Robert Ménard

    1. , Malaria Biology and Genetics Unit, Institut Pasteur, Paris, France

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  • Presents definitive protocols for malaria researchers
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 923)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xv
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  2. Parasite Culturing

    1. Front Matter

      Pages 1-1
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  3. Parasite Culturing

    1. In Vitro Culturing Plasmodium falciparum Erythrocytic Stages

      • Alexander G. Maier, Melanie Rug
      Pages 3-15

    2. Production of Plasmodium falciparum Gametocytes In Vitro

      • Kathryn Shaw Saliba, Marcelo Jacobs-Lorena
      Pages 17-25

    3. In Vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes

      • Anil K. Ghosh, Marcelo Jacobs-Lorena
      Pages 27-33

    4. Ex Vivo Culture of Plasmodium Vivax and Plasmodium Cynomolgi and In Vitro Culture of Plasmodium Knowlesi Blood Stages

      • Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, Clemens H. M. Kocken
      Pages 35-49

    5. Laboratory Maintenance of Rodent Malaria Parasites

      • Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker et al.
      Pages 51-72

  4. Genome Manipulation

    1. Front Matter

      Pages 73-73
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  5. Genome Manipulation

    1. Transfection of Plasmodium falciparum

      • Melanie Rug, Alexander G. Maier
      Pages 75-98

    2. Transfection of Rodent Malaria Parasites

      • Nisha Philip, Rachael Orr, Andrew P. Waters
      Pages 99-125

    3. Recombination-Mediated Genetic Engineering of Plasmodium berghei DNA

      • Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, Oliver Billker
      Pages 127-138

    4. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database

      • Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, Chris J. Janse
      Pages 139-150

    5. Transient Transfection of Plasmodium vivax Blood-Stage Parasites

      • Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer
      Pages 151-159

    6. Silencing of Genes and Alleles by RNAi in Anopheles gambiae

      • Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, Stéphanie A. Blandin
      Pages 161-176

  6. “OMIC” Techniques

    1. Front Matter

      Pages 177-177
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  7. OMIC’ Techniques

    1. Analysis of Variant Gene Family Expression by Quantitative PCR

      • Sohini Sanyal, Thomas J. Templeton, Cristina K. Moreira
      Pages 179-188

    2. DNA Microarray-Based Genome-Wide Analyses of Plasmodium Parasites

      • Zbynek Bozdech, Sachel Mok, Archna P. Gupta
      Pages 189-211

    3. Whole-Genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform

      • Heather J. Painter, Lindsey M. Altenhofen, Björn F. C. Kafsack, Manuel Llinás
      Pages 213-219

    4. Transcriptome Analysis Using RNA-Seq

      • Wieteke A. M. Hoeijmakers, Richárd Bártfai, Hendrik G. Stunnenberg
      Pages 221-239

    5. Experimental Tools for the Study of Protein Phosphorylation in Plasmodium

      • Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, Christian Doerig
      Pages 241-257
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Keywords

About this book

Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of  Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and  techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical,  Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory  researchers.

Editors and Affiliations

  • , Malaria Biology and Genetics Unit, Institut Pasteur, Paris, France

    Robert Ménard

Bibliographic Information

  • Book Title: Malaria

  • Book Subtitle: Methods and Protocols

  • Editors: Robert Ménard

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-026-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-025-0Published: 19 September 2012

  • Softcover ISBN: 978-1-4939-5958-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-026-7Published: 19 September 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 626

  • Topics: Infectious Diseases, Parasitology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

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