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  • © 2012

Plant Salt Tolerance

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 913)

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Table of contents (28 protocols)

  1. Biochemical Assays

  2. Molecular Techniques

    1. Front Matter

      Pages 263-263
    2. Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Specific Responses to Salinity Stress in Arabidopsis and Rice

      • Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, Alexander A. T. Johnson
      Pages 265-276
    3. Transformation Using Controlled cDNA Overexpression System

      • Gábor Rigó, Csaba Papdi, László Szabados
      Pages 277-290
    4. Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress

      • Prasad Senadheera, Frans J. M. Maathuis
      Pages 291-303
    5. Marker-Assisted Selection in Plant Breeding for Salinity Tolerance

      • M. Ashraf, N. A. Akram, Mehboob-ur-Rahman, M. R. Foolad
      Pages 305-333
    6. Transcriptomics on Small Samples

      • Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo, Asmini Athman, Matthew Gilliham
      Pages 335-350
    7. Plastid Transformation for Abiotic Stress Tolerance in Plants

      • K. C. Bansal, A. K. Singh, S. H. Wani
      Pages 351-358
    8. Manipulating Expression of Tonoplast Transporters

      • Zhigang Li, Man Zhou, Qian Hu, Shane Reighard, Shuangrong Yuan, Ning Yuan et al.
      Pages 359-369
    9. Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities

      • Alonso Rodríguez, Begoña Benito, Olivier Cagnac
      Pages 371-386
  3. Other Methods

    1. Front Matter

      Pages 387-387
    2. Trait Dissection of Salinity Tolerance with Plant Phenomics

      • Bettina Berger, Bas de Regt, Mark Tester
      Pages 399-413
    3. Measuring Soil Salinity

      • Marcus Hardie, Richard Doyle
      Pages 415-425
  4. Back Matter

    Pages 427-432

About this book

Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.

Editors and Affiliations

  • School of Agricultural Science, University of Tasmania, Hobart, Australia

    Sergey Shabala

  • INRA-SupAgro, BPMP-IBIPGC, Montpellier, France

    Tracey Ann Cuin

Bibliographic Information

  • Book Title: Plant Salt Tolerance

  • Book Subtitle: Methods and Protocols

  • Editors: Sergey Shabala, Tracey Ann Cuin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-986-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-985-3Published: 16 August 2012

  • Softcover ISBN: 978-1-4939-5939-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-986-0Published: 16 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 432

  • Number of Illustrations: 30 b/w illustrations, 26 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access