Antibody Engineering

Methods and Protocols, Second Edition

  • Patrick Chames

Part of the Methods in Molecular Biology book series (MIMB, volume 907)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. In Silico Methods

    1. Front Matter
      Pages 1-1
    2. Marie-Paule Lefranc, François Ehrenmann, Chantal Ginestoux, Véronique Giudicelli, Patrice Duroux
      Pages 3-37
  3. Generation of Diversity

    1. Front Matter
      Pages 57-57
    2. Sylvie Peraldi-Roux
      Pages 73-83
    3. Michael Hust, André Frenzel, Torsten Meyer, Thomas Schirrmann, Stefan Dübel
      Pages 85-107
    4. Gautier Robin, Pierre Martineau
      Pages 109-122
    5. Eeva-Christine Brockmann
      Pages 123-144
    6. Cécile Vincke, Carlos Gutiérrez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, Serge Muyldermans
      Pages 145-176
    7. Mischa Roland Müller, Ronan O’Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, Caroline Jane Barelle
      Pages 177-194
    8. Romain Rouet, Kip Dudgeon, Daniel Christ
      Pages 195-209
  4. Selections of Lead Candidate

    1. Front Matter
      Pages 211-211
    2. Julie Matz, Patrick Chames
      Pages 213-224
    3. Klervi Even-Desrumeaux, Patrick Chames
      Pages 225-235
    4. Sang Jick Kim, Hyo Jeong Hong
      Pages 247-257
    5. Nathalie Scholler
      Pages 259-280
    6. Bryan M. Edwards, Mingyue He
      Pages 281-292

About this book

Introduction

More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an average rate of 50 per year and that is predicted to continue well into the future. Notwithstanding the many achievements already made in the field, there is still a lot of room for improvements for these molecules in terms of activity, and a plethora of approaches have been attempted to optimize these molecules. Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Antibody Engineering: Methods and Protocols, Second Edition serves as an invaluable resource for both experts and those new to the field, and most of all as a source of inspiration for the creation of the antibodies of tomorrow.

Keywords

Fc engineering antibody repertoires antibody variable domains in silico methods recombinant antibodies therapeutic antibodies

Editors and affiliations

  • Patrick Chames
    • 1
  1. 1.Antibody Therapeutics & ImmunotargetingINSERM U624Marseille CEDEX 09France

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-974-7
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-973-0
  • Online ISBN 978-1-61779-974-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book

Industry Sectors