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Single Domain Antibodies

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Dirk Saerens

    1. , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Brussels, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Serge Muyldermans

    1. , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Brussels, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Features methods of selection, expression, improvement, and library construction of single domain antibodies
  • Includes step-by-step instruction that has been tested in labs around the world
  • Provides key tips and implementation advice that is essential for success
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 911)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xv
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  2. Overview of Single Domain Antibodies

    1. Front Matter

      Pages 1-1
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    2. From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small

      • Jean-Luc Teillaud
      Pages 3-13

    3. Introduction to Heavy Chain Antibodies and Derived Nanobodies

      • Cécile Vincke, Serge Muyldermans
      Pages 15-26

    4. Overview and Discovery of IgNARs and Generation of VNARs

      • Stewart D. Nuttall
      Pages 27-36

  3. Single Domain Antibody Library Construction

    1. Front Matter

      Pages 37-37
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    2. Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies

      • Olga Ignatovich, Laurent Jespers, Ian M. Tomlinson, Ruud M. T. de Wildt
      Pages 39-63

    3. Preparation of a Naïve Library of Camelid Single Domain Antibodies

      • Aurelien Olichon, Ario de Marco
      Pages 65-78

  4. Selection of Single Domain Antibodies

    1. Front Matter

      Pages 79-79
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    2. Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation

      • Peter Verheesen, Toon Laeremans
      Pages 81-104

    3. Semiautomated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens

      • Jyothi Kumaran, C. Roger MacKenzie, Mehdi Arbabi-Ghahroudi
      Pages 105-124

    4. Pichia Surface Display: A Tool for Screening Single Domain Antibodies

      • Kristof De Schutter, Nico Callewaert
      Pages 125-134

    5. Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method

      • Mireille Pellis, Serge Muyldermans, Cécile Vincke
      Pages 135-150

    6. Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

      • Tomoyuki Tanaka, Terence H. Rabbitts
      Pages 151-173

    7. Selection of Functional Single Domain Antibody Fragments for Interfering with Protein–Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System

      • Tomoyuki Tanaka, Terence H. Rabbitts
      Pages 175-182

    8. Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization

      • Armin Sepp, Andrew Griffiths
      Pages 183-198

    9. Selection of VHHs Under Application Conditions

      • Edward Dolk, Theo Verrips, Hans de Haard
      Pages 199-209

    10. Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies

      • Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie, Jamshid Tanha
      Pages 211-239

    11. Selection of VHH Antibody Fragments That Recognize Different Aβ Depositions Using Complex Immune Libraries

      • Rinse Klooster, Kim S. Rutgers, Silvère M. van der Maarel
      Pages 241-253

  5. Expression of Single Domain Antibodies and Derivatives

    1. Front Matter

      Pages 225-225
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Keywords

About this book

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Brussels, Belgium

    Dirk Saerens, Serge Muyldermans

Bibliographic Information

  • Book Title: Single Domain Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Dirk Saerens, Serge Muyldermans

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-968-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-967-9Published: 11 August 2012

  • Softcover ISBN: 978-1-4939-5915-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-968-6Published: 11 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 580

  • Number of Illustrations: 54 b/w illustrations, 35 illustrations in colour

  • Topics: Immunology, Antibodies

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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