Antiviral Resistance in Plants

Methods and Protocols

  • John M. Watson
  • Ming-Bo Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 894)

Table of contents

  1. Front Matter
    Pages i-xi
  2. John A. Lindbo
    Pages 1-16
  3. Claire Agius, Andrew L. Eamens, Anthony A. Millar, John M. Watson, Ming-Bo Wang
    Pages 17-38
  4. Changyong Zhou, Yan Zhou
    Pages 69-81
  5. Shawn Bachan, Savithramma P. Dinesh-Kumar
    Pages 83-92
  6. Hajime Yaegashi, Masamichi Isogai, Nobuyuki Yoshikawa
    Pages 113-122
  7. Qian-Hao Zhu, Christopher A. Helliwell
    Pages 123-137
  8. Robert S. Anderssen, Peter M. Waterhouse
    Pages 139-154
  9. Neil A. Smith, Andrew L. Eamens
    Pages 155-172
  10. Shaun J. Curtin, Agustín Zsögön, John M. Watson, Peter M. Waterhouse
    Pages 173-189
  11. Neena Mitter, Ralf G. Dietzgen
    Pages 191-208
  12. Jing Qu, Jian Ye, Rongxiang Fang
    Pages 209-222
  13. H. Alexander Ebhardt, Mariana Oviedo Ovando, Peter J. Unrau
    Pages 223-239
  14. Robert A. Owens, Teruo Sano, Nuria Duran-Vila
    Pages 253-271
  15. Aardra Kachroo, Said Ghabrial
    Pages 287-297

About this book

Introduction

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.  These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

Keywords

Antiviral Resistance Pathogen-mediated virus resistance Plant viral infections Plant-virus interactions RNA silencing Sub-viral RNAs Virus-induced gene silencing

Editors and affiliations

  • John M. Watson
    • 1
  • Ming-Bo Wang
    • 2
  1. 1.Division of Plant IndustryCSIROCanberraAustralia
  2. 2.Division of Plant IndustryCSIROCanberraAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-882-5
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-881-8
  • Online ISBN 978-1-61779-882-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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