Table of contents
About this book
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-61779-770-5
- Copyright Information Springer Science+business Media, LLC 2012
- Publisher Name Humana Press
- eBook Packages Springer Protocols
- Print ISBN 978-1-61779-769-9
- Online ISBN 978-1-61779-770-5
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
- About this book