Single-Cell Analysis

Methods and Protocols

  • Sara Lindström
  • Helene Andersson-Svahn

Part of the Methods in Molecular Biology book series (MIMB, volume 853)

Table of contents

  1. Front Matter
    Pages i-x
  2. Dino Di Carlo, Henry Tat Kwong Tse, Daniel R. Gossett
    Pages 1-10
  3. Conventional Methods

  4. Miniaturized Solutions

    1. Front Matter
      Pages 39-39
    2. Sara Lindström, Helene Andersson-Svahn
      Pages 41-52
    3. Michael D. Vahey, Joel Voldman
      Pages 53-63
    4. Séverine Le Gac, Albert van den Berg
      Pages 65-82
    5. Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, Atsushi Muraguchi
      Pages 141-150
    6. Elsa Correia Faria, Peter Gardner
      Pages 151-167
    7. Yan Zhang
      Pages 169-176
    8. Martin Wiklund, Björn Önfelt
      Pages 177-196
  5. Reviews on Chosen Subjects Within the Field

    1. Front Matter
      Pages 197-197
    2. Soo Hyeon Kim, Dominique Fourmy, Teruo Fujii
      Pages 199-210
    3. Ayça Yalçın, Yvonne J. Yamanaka, J. Christopher Love
      Pages 211-235

About this book

Introduction

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail.  The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.

Keywords

Cellular behaviour Individual cells Miniaturization Miniaturized systems Single cell analysis

Editors and affiliations

  • Sara Lindström
    • 1
  • Helene Andersson-Svahn
    • 2
  1. 1.POOLIA SwedenStockholmSweden
  2. 2., Division of NanobiotechnologyRoyal Institute of TechnologyStockholmSweden

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-567-1
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-566-4
  • Online ISBN 978-1-61779-567-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma